作　　者： ; ; (沈文英）; (马广智）;

机构地区： 中山大学生命科学学院生物系

出　　处： 《中山大学学报论丛》 1995年第1期153-156,共4页

摘　　要： 草鱼生长激素cDNA在大肠杆菌细胞中表达后，重组草鱼生长激素在胞内形成不溶性的包含体．这种包含体在洗涤、变性、复性和C12反相层析等步骤后，产物再经快速蛋白液相色谱，重组草鱼生长激素在SDS-聚丙烯酰凝胶电泳中显示均一性，纯度可达90％放射免疫分析表明：重组草鱼生长激素具有草鱼生长激素的免疫活住．分离纯化产率约为1L细菌培养液1～2m重组草鱼生长激素． Cloned cDNA of grass carn growth hormone (rgGH) was expressed in Escherichia coli cells and the rgGH was accumulated as insoluble inclusion theies. The inclusion bodies were washed and denatured. After renaturation the rgGH was purified by C15 revcrse phase chromatography and fast protein liquid chromatography (FPLC) to 90% purity. The results of radioimmunoassay show that the rgGH possesses immunoreatctivity of growth hormone. The final yield of the rgGH was 1 ~ 2 mg per liter of bacteria culture.

关 键 词： 大肠杆菌 包含体 基因重组草鱼生长激素

领　　域： [文化科学] [文化科学]