作　　者： ; ; ;

机构地区： 华南农业大学兽医学院

出　　处： 《中国兽医学报》 2017年第1期144-147,189,共5页

摘　　要： 选用浓度为30,60,120,180,240μmol/L的氯化铜(CuCl_2)作用于原代大鼠脑微血管内皮细胞(BMEC)12h,测定细胞活力、细胞内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和诱导型一氧化氮合酶(iNOS)活性的变化。结果显示:与对照组相比,细胞活力明显降低,30μmol/L浓度组SOD的活性变化不明显,60μmol/L和120μmol/L浓度组SOD的活性升高,而180μmol/L和240μmol/L浓度组SOD的活性降低,CAT的活性均明显降低,iNOS的活性均明显升高。结果表明:铜过量可致BMEC损伤,而氧化应激是其中一个重要因素。 Primary rat brain microvascular endothelial cells （BMEC） were incubated with CuCl2 （30,60,120,180 and 240 μmol/L） for 12 h. Cell viability, activities of cellular superoxidase dis- mutase （SOD）,catalase （CAT） and inducible nitric oxide synthase （iNOS） were measured after incubation. Results showed that cell viability was decreased significantly, SOD activity had almost no change under the concentration of 30 μmol/L,while it increased under the concentration of 60 and 120 μmol/L and decreased under the concentration of 180 and 240 μmol/L. CAT activity de- creased and iNOS activity increased significantly, compared to that of the controls. The conse- quences suggested that excess copper affects the cell viability of BMEC, and oxidative stress could be an essential factor.

关 键 词： 铜 大鼠脑微血管内皮细胞 氧化应激

领　　域： [农业科学] [农业科学] [农业科学] [农业科学] [农业科学] [农业科学]