作　　者： ; ; ; ; ; ;

机构地区： 广州中医药大学

出　　处： 《中国实验动物学报》 2016年第5期526-528,545,共4页

摘　　要： 目的观察NOD/SCID小鼠动情周期及卵巢切除术后阴道涂片的变化。方法连续观察9 d NOD/SCID鼠,每日进行两次阴道涂片,计算动情周期时间及发生率。卵巢切除后,阴道涂片并观察阴道涂片变化。结果NOD/SCID小鼠动情周期为4-6 d。有规律动情的小鼠占80%。阴道口状态与动情周期无明显相关。卵巢切除术后,阴道涂片呈动情后期或动情间期改变。结论采用阴道脱落细胞涂片法可判断NOD/SCID雌性小鼠的动情周期及特点,NOD/SCID雌性小鼠卵巢切除手术有效。 Objective To observe the changes in estrous cycle and vaginal smears in ovarectomized NOD/SCID mice. Methods To continuously observe the estrous cycle time by vaginal smears of NOD/SCID mice in consecutive nine days,twice daily. After ovariectomy,the changes of estrous cycle were observed by vaginal smears for 7 days. Results The estrous cycle in NOD/SCID mice was 4-6 days. Regular estrous mice accounted for 80%. There was no significant correlation between vaginal opening and estrous cycle status. After ovariectomy,the vaginal smears showed characteristics of metestrus or diestrus. Conclusions Vaginal smear cytology can be used to determine the estrous cycle and characteristics of NOD/SCID female mice. The ovariectomized operation of NOD/SCID female mice is effective.

关 键 词： 小鼠 动情周期 阴道涂片 卵巢切除术

领　　域： [生物学]