作　　者： ; ; ; ; ; ; ; ;

机构地区： 佛山科学技术学院生命科学学院园艺系

出　　处： 《植物营养与肥料学报》 2016年第3期729-735,共7页

摘　　要： [目的]研究豌豆不同品种耐铝性和根尖根段耐铝性与果胶及其甲基酯化间的关系,为进一步揭示植物耐铝机理以及耐铝性状的遗传改良提供依据。[方法]以豌豆品种Hyogo和Alaska为试验材料,采用Hoagland培养方式,测定了不同品种不同根段果胶含量、果胶甲基酯化度和果胶甲酯酶活性,研究了其差异及原因。[结果]在15和30μmol/L铝浓度胁迫条件下,豌豆品种Alaska根相对伸长率均显著高于品种Hyogo,同时有根尖0~5 mm和5~10 mm段有更少的胼胝质生成和累积在30μmol/L浓度下不同根段间均达到显著差异,同时品种Hyogo根尖0~2.5 mm和2.5~5.0 mm段铝含量均显著高于品种Alaska,说明品种Alaska和品种Hyogo间存在耐铝性差异,其中品种Alaska耐铝性高于品种Hyogo,即品种Hyogo为铝敏感品种,品种Alaska是耐铝品种。比较两者不同根段（0~2.5 mm、2.5~5.0 mm和5.0~10.0 mm）的铝含量与果胶含量、果胶甲基酯化度、PME活性间的关系发现耐铝品种不同根段中的铝含量均小于敏感品种,并且在0~2.5 mm和2.5~5.0 mm段间达到显著性差异;根尖不同根段果胶糖醛酸含量大小依次为0~2.5〉2.5~5.0〉5.0~10.0 mm,耐铝品种Alaska根尖细胞壁果胶和未甲酯化果胶含量均显著低于Hyogo,并且0~2.5 mm根段差异最大。根尖不同根段果胶甲基酯化度从根尖向上逐渐降低,并且耐铝品种Alaska高于铝敏感品种Hyogo,其中0~2.5 mm段间的差异达到显著水平;在对两个品种果胶甲基酯化酶（PME）活性进一步分析发现,PME活性大小依次为0~2.5〉2.5~5.0〉5.0~10.0 mm,两品种0~2.5 mm和2.5~5.0 mm根段间均达到显著差异。[结论]铝敏感品种Hyogo在0~2.5 mm和2.5~5.0mm根段具有较高PME活性和较低果胶甲基酯化程度。豌豆根尖果胶含量和甲基酯化度尤其是0~2.5 mm根段是豌豆耐铝性差异的重要原因;Alaska根尖细胞壁的果胶含量低和果胶甲基酯化度高（尤其是0~2.5 mm段）是其耐铝的重要机 【Objectives】The study investigated the relationship between changes of cell wall pectin,pectin methyl esterification degree and aluminum tolerance in different cultivars of pea（ Pisum sativum） to provide theoretical evidences how genetic differences affected aluminum tolerance during plant growth. 【Methods】All cultivars were cultivated by mist under 24℃ growth chamber for 24 h after seed soaking. The root was divided into threesegments： 0-2. 5 mm,2. 5- 5. 0 mm and 5. 0- 10. 0 mm. Pectin content,methyl esterification degree and the activity of pectin methylesterase（ PME） were measured in different root segments of cultivar Hyogo and cultivar Alaska. Relative elongation rate（ RER）,callose and Al content were also tested to compare Al tolerance in different root zones and cultivars of pea. 【Results】The RER of Alaska cultivar were significantly higher than Hyogo under Al concentration of 15 μmol / L and 30 μmol / L. And,the callose and Al of 0- 5 mm or 5- 10 mm segments in Alaska cultivar were accumulated much less than Hyogo. This result confirmed that the Alaska was an Al resistant cultivar and Hyogo was an Al sensitive cultivar. Alaska showed lower Al contents in all segments. In the meantime,the different Al levels between two species were showed significantly in 2. 5- 5. 0 mm and 5. 0-10. 0 mm segments. Uronic acid content in cell wall pectin was found decreasing sequentially from segment 0-2. 5mm to 5. 0-10. 0 mm in both pea species. Alaska was measured lower cell wall pectin and un-methylated pectin compared with Hyogo,especially in segment 0- 2. 5 mm. The activity of PME also decreased successively from segment 0- 2. 5 mm to 5. 0- 10. 0 mm in both Alaska and Hyogo. The degree of pectin methylation was significantly higher in 0-2. 5 mm root segment than the other root segments in Alaska. Alaska had relatively lower content of uronic acid,lower activity of PME,and higher degree of pectin methylation,especially in 0- 2. 5 mm root segment. 【Conclusions】The key factor for pea cult

关 键 词： 豌豆 耐铝性 细胞壁果胶 甲基酯化度 果胶甲基酯化酶

领　　域： [生物学]