作　　者： ; ; ; ; ; ; ; (荆喜中）; (凌雪荧）; (李韵峰）;

机构地区： 广东省实验动物监测所

出　　处： 《中国实验动物学报》 2016年第2期158-163,共6页

摘　　要： 目的建立小鼠脓毒症模型并进行评价,为研究脓毒症致病机制和开发抗炎药物提供模型动物。方法采用盲肠结扎穿孔法(cecal ligation and puncture,CLP)诱导小鼠脓毒症,通过动物生存、术后小鼠载菌量、血常规和血生化指标、细胞因子水平、组织病理变化等方面对模型进行评价。结果小鼠的死亡率与盲肠结扎部位密切相关,盲肠结扎50%小鼠12 d存活率在40%左右,结扎75%小鼠4 d全部死亡(P<0.01)。与假手术组相比,50%结扎CLP小鼠血液和腹腔中载菌量增加,白细胞下降,差异有显著性(P<0.001)。CLP小鼠肝转氨酶ALT、AST和血清尿素氮BUN水平升高,差异具有显著性(P<0.01),炎症因子IL1α、IL6、IL10、MIP1α、MIP1β、TNFα水平升高。手术后48 h小鼠的肝和肺出现明显组织病理损伤。结论小鼠CLP模型具有典型的脓毒症病理特征,为后期研究抗炎药物的筛选提供了较好的动物模型。 Objective The purpose of this study was to establish and evaluate a mouse model of sepsis for studying the mechanism of sepsis and development of anti-inflammatory drugs. Methods The sepsis in mice was induced by cecal ligation and puncture（ CLP）. The survival rates,microbial load,liver and kidney damages,cytokines and pathological changes were detected to evaluate the mouse models. Results The death of mice was closely related with the ligated sites.The mice with 50% cecal ligation displayed about 40% of 12-day survival rate,however,all the mice with 75% cecum ligation died within 4 days（ P〈0. 01）. Compared with the sham surgery group,the mice with 50% cecal ligation had a high microbial load in the blood and abdominal cavity. Leukopenia was also emerged（ P〈0. 001）. CLP mice demonstrated elevated levels of serum ALT,AST and BUN（ P〈0. 01）. The levels of IL1α,IL6,IL10,MIP1α,MIP1β,and TNFα were increased a lot. The liver and lung showed obvious pathological injury at 48 h post CLP. Conclusions The established mouse model of CLP shows typical characteristics of sepsis and is an ideal tool for further study of anti-inflammatory drugs.

关 键 词： 脓毒症 盲肠结扎穿孔 小鼠模型 炎症反应 感染

领　　域： [生物学]