作　　者： ; ; ; (任佳明）;

机构地区： 贵州大学

出　　处： 《中国酿造》 2016年第4期24-28,共5页

摘　　要： 实验优化了黑曲霉B1401液态发酵直接投加单宁酸生物法制备没食子酸的工艺条件,得出的最佳工艺条件为:底物单宁酸添加方式为分批添加,其累计添加质量浓度达204 mg/m L;转化过程p H维持在4.0;转化温度为30℃;转化过程摇瓶转速为160 r/min。将优化后的工艺条件放大到5 L发酵罐,单宁酸转化效率优于摇瓶发酵,转化平衡时间由摇瓶的48 h缩短至44 h,没食子酸的产率由70.35%提高至74.04%,质量浓度由144 mg/m L提高至161 mg/m L。 The optimal technological conditions of gallic acid preparation from tannic acid was studied in the submerged fermentation by Aspergillus niger B1401. Results showed that the fed-batch adding tannin method was better. The optimum process conditions were obtained as follows： total adding tannins concentration 204 mg/ml, pH 4.0, reactive temperature 30℃, and rotate speed 160 r/min. At the same conditions, the experiments were carried out in 5 L automatic fermentor, the conversion efficiency was better than that in shake flask fermentation. The transformation balance time was shortened to 44 h from 48 h, the yield of gallic acid increased from 70.35% to 74.04%, and the gallic acid concentration increased from 144 mg/ml to 161 mg/ml.

关 键 词： 黑曲霉 单宁酶 生物转化 没食子酸

领　　域： [轻工技术与工程]