作　　者： ; ; ; ; ; ; ; (郑至嘉）; (田靖琳）;

机构地区： 深圳大学

出　　处： 《癌变．畸变．突变》 2016年第1期19-22,共4页

摘　　要： 目的:通过建立体外模型研究磷化铟/硫化锌(InP/ZnS)量子点对巨噬细胞功能的影响。方法:以不同浓度的InP/ZnS量子点作用于小鼠腹腔巨噬细胞RAW264.7不同时间后,采用荧光显微镜观察巨噬细胞对量子点的摄取情况;用CCK-8法检测量子点对巨噬细胞增殖能力的影响;用酶联免疫(ELISA)法检测量子点及免疫原CpG寡脱氧核苷酸(CpG-ODN)对肿瘤坏死因子-α(TNF-α)和白介素-6(IL-6)分泌的影响。结果:与阴性对照组比较,2.5、5和10μg/mL InP/ZnS量子点处理4 h后可被巨噬细胞摄取并定位于细胞浆内;当InP/ZnS量子点浓度达到5μg/mL以上时,作用24和48 h后细胞增殖能力随量子点浓度增加而明显下降(P<0.05和P<0.01);经量子点处理的巨噬细胞在有或无CpG-ODN的刺激下,TNF-α的分泌出现不同程度增加(P<0.01),而IL-6变化不明显(P>0.05)。结论:InP/ZnS量子点可以被巨噬细胞摄取,抑制巨噬细胞的增殖能力,并促进TNF-α的分泌。 OBJECTIVE：To investigate the effects of InP/ZnS quantum dots（InP/ZnS QDs） on function of macrophages using in vitro cell model.METHODS：The mouse macrophage RAW264.7 cells were exposed to different concentrations of InP/ZnS QDs.The uptake of InP/ZnS QDs by macrophages was observed under a fluorescence microscope4 hours later.Effect of InP/ZnS QDs on cell proliferation was assessed by CCK-8 assay,and the levels of interleukin-6（IL-6） and tumor necrosis factor-a（TNF-a） released by macrophages were determined by ELISA assay.RESULTS：InP/ZnS QDs entered the cells and remained mostly in die cytoplasm in 4 hours.Cells exposed to InP/ZnS QDs showed significantly decreased cell reproduction 24 hours and 48 hours lateT（P〈0.05,P〈0.01）,and the levels of TNF-a released with or without CpG-ODN stimulationby macrophages significantly increased 24 hours later（i0.01）,but the levels of IL-6 remained unchanged.In addition,InP/ZnS QDs exposure led to increased release of TNF-a by macrophages following re-stimulation with CpG-ODN.CONCLUSION：The results suggest that InP/ZnS QDs could be taken up by macrophages,and could inhibit the cell viability and increase release of TNF-a.

关 键 词： 磷化铟 硫化锌 量子点 巨噬细胞 细胞毒性 细胞因子

领　　域： [生物学]