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舍内不同氨气浓度对肉鸡抗氧化性能及肉品质的影响
Effect of Ammonia in Broiler Houses on the Antioxidant Activity and Meat Quality of Broiler

作  者: ; ; ; ; (栾素军);

机构地区: 中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室

出  处: 《中国农业科学》 2015年第21期4347-4357,共11页

摘  要: [目的]探讨鸡舍内不同氨气浓度对肉鸡抗氧化性能和肉质性状的影响。[方法]采用单因素完全随机设计,将400只21日龄健康艾拔益加(AA)肉公鸡,随机分在4个呼吸代谢舱中,每个舱为1个处理,每个处理设4个重复,每个重复25只鸡。4个处理组分别为:对照组氨气浓度控制在<3 mg·kg-1,试验组氨气浓度分别控制在(25±3)、(50±3)和(75±3)mg·kg-1。呼吸代谢舱采用全自动化控制养殖环境条件。试验期为21 d,21—31 d为试验前期,32—42 d为试验后期。肉鸡采用网上平养,自由采食和饮水。分别在32 d和42 d屠宰取样,测定肉鸡血液、肌肉、肝脏的抗氧化性能以及肉质性状。[结果](1)抗氧化性能:在21—31日龄阶段,随着鸡舍氨气浓度的升高,肉鸡血清超氧化物歧化酶(superoxide dismutase,SOD)活性呈二次曲线增加(P<0.05),总抗氧化能力(total antioxidation capacity,T-AOC)活性和丙二醛(malondialdehyde,MDA)含量呈线性增加(P<0.05);胸肌过氧化氢酶(catalase,CAT)活性和MDA含量具有呈线性增加的趋势(0.05 [Objective]The aim of this experiment is to study the effects of different concentration of ammonia on the antioxidant capacity and the meat quality of broiler chickens.[Method] The study has adopted a single factor completely randomized design.A total of 400 21-day-old Arbor Acres(AA) male broilers were randomly divided into 4 respiratory chambers,and each chamber was a treatment with 4 replicates,and 25 broilers in each replicate.The following concentrations of ammonia were set in different groups:〈3 mg·kg^-1(control group),(25±3),(50±3),(75±3) mg·kg^-1 respectively.The respiratory chambers controlled the environment with full-automation.The entire experimental period was 21 days:the starter phase was 21-31 d,and the later phase was 32-42 d.Broilers were provided ad libitum access to feed and water in nets.The antioxidant capacity of blood and muscle,and meat quality were determined at 32 d and 42 d of age.[Result](1) Antioxidant capacity:At 21-31 d of age,with ammonia concentration increasing,the activity of SOD showed a quadratic increase(P〈0.05),and the activity of T-AOC(total antioxidation capacity) and the content of MDA(malondialdehyde) showed a linear increase(P〈0.05) in serum.The activity of CAT(catalase) and the content of MDA in the breast muscle showed a linear increase trend(0.05〈P〈0.10),and the activity of T-AOC showed a linear decrease(P〈0.05) in breast muscle.The activity of T-AOC showed a linear increase trend(0.05〈P〈0.10) in thigh muscle of broilers,and the activity of SOD(superoxide dismutase) of broilers in 75 mg·kg^-1 group was higher than in others(P〈0.05).The activity of CAT in liver showed a linear decrease trend(0.05〈P〈0.10),the content of MDA showed a linear increase(P〈0.05).At 32-42 d of age,with ammonia concentration increasing,the activity of GSH-Px(glutathione peroxidase) showed a quadratic decrease(P〈0.05),and the activity of CAT showed a linear decrease in serum of

关 键 词: 氨气 抗氧化 肉品质 肉鸡

领  域: [农业科学] [农业科学]

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