作　　者： ; ; ; ; ;

机构地区： 中国科学院大连化学物理研究所

出　　处： 《中国科学：生命科学》 2015年第10期969-975,共7页

摘　　要： 氧化还原反应是最常见的代谢反应类型之一,其中绝大部分通过辅因子依赖型氧化还原酶催化实现.由于辅因子广泛参与细胞内氧化还原反应及其他生物学过程,因代谢途径改造而扰动辅因子水平的生物学效应尚难以预测.设计构建基于人工辅因子的正交体系,是减少人工代谢途径与内源代谢网络相互干扰、降低系统复杂度、提高调控代谢网络有效性的新策略.本文探讨了正交氧化还原体系的构建方法,并结合实例说明其对提高能量传递特异性和人工代谢途径效率的重要意义. Redox chemistry constitutes one of the most important metabolic processes. The majority of enzymes that catalyze redox reactions are cofactor-dependent. Because cofactors are involved in many metabolic reactions as well as other biological events, it remains challenging to predict the consequences of cofactor level manipulation. It is believed that an orthogonal redox system may help reduce the system＇s complexity and improve the effectiveness of metabolic control by avoiding interactions between artificial and natural systems. Here, we reviewed the advances in developing orthogonal redox systems. Some interesting examples were selected to illustrate the mechanism by which orthogonal redox systems can be applied for pathway-selective electron transmission and more effective metabolic control.

关 键 词： 正交氧化还原 合成生物学 化学生物学 辅因子 代谢工程

领　　域： [理学] [理学]