作　　者： ; ; ; ;

机构地区： 新疆农业科学院

出　　处： 《西北植物学报》 2015年第10期1972-1977,共6页

摘　　要： 该研究以陆地棉‘徐州142’为材料,于开花后5、10、15、20、25、30d分别取样(棉铃)代表6个不同时期的棉纤维,利用双向电泳技术比较了棉纤维发育不同时期蛋白质组的变化,以明确不同发育阶段差异表达蛋白质及其功能与纤维发育之间的关系,为棉花产量和品质的改良提供理论依据。结果显示:(1)随着棉花的发育,棉纤维中的总蛋白含量逐渐降低,在开花后5d(5DPA)时棉花纤维中总蛋白的含量最高,达到11.7%,同时2-DE图谱中发现了15个明显的差异蛋白点;(2)运用MALDI-TOF-MS对差异蛋白质点鉴定结果发现,有5个明显的差异蛋白与棉花纤维发育相关,分别是GhSAM、GhPGK、GhCSD、GhFB和GhMDH蛋白;(3)开花后不同时期棉纤维蛋白质组的2-DE图谱表明,GhSAM、GhPGK1和GhMDH 3个蛋白在整个发育期都表达,但GhCSD从15DPA开始表达,GhFB从20DPA开始表达,而且GhSAM在15DPA时蛋白表达量最高,GhPGK1在10DPA时表达量最高,GhMDH、GhCSD和GhFB都是在30DPA时表达量最高。研究表明,棉纤维发育不同时期存在着差异蛋白,且在纤维发育不同时期蛋白的表达量也存在差异,同时这些差异蛋白参与能量代谢、碳代谢、细胞周期调控和发育等途径。 In this study,the cotton fiber protein of‘Xuzhou 142＇have been performed to exhibit the protein profile differences between different development stages of cotton fiber cells.1）The total protein content was decreased gradually,and the content was the highest in 5DPA.Meanwhile,15 distinct protein spots were found in the 2-DE map;2）It was found that there are five distinct different proteins associated with cotton fiber development with MALDI-TOF-MS：They were GhSAM,GhPGK,GhCSD,GhFB and GhMDH protein,respectively;3）The 2-DE map analysis of cotton fiber in different development stages showed that GhSAM,GhPGK1 and GhMDH were expressed throughout the whole development stage,GhCSD was expressed from 15 DPA,GhFB was expressed from 20 DPA,and GhSAM expression was higher at 15 DPA,GhPGK1expression was higher at 10 DPA,GhMDH,GhCSD and GhFB expression were higher at 30 DPA.The results showed that the differences were existed in different cotton fiber developments stages,and these proteins are involved in a wide range of physiological processes,such as energy metabolism,carbohydrate metabolism,cell cycle control and development.

关 键 词： 棉花纤维 蛋白质组 表达量

领　　域： [生物学] [生物学]