作　　者： ; ; ; ; ;

机构地区： 云南省林业科学院

出　　处： 《西部林业科学》 2015年第5期57-61,75,共6页

摘　　要： 肉桂酰辅酶A还原酶(Cinnamoyl-CoA reductase,CCR)是苯丙烷类代谢途径中的关键酶,在七彩红竹木质素和花青素合成代谢流向中起重要作用。依据七彩红竹转录组数据设计特异引物,采用反转录PCR技术从七彩红竹中克隆得到一个新的CCR基因的全长cDNA序列,命名为IhCCR-1(登录号:KP271440)。结果表明,该基因全长cDNA为1 038 bp,编码345个氨基酸的蛋白质,属于酸性稳定蛋白,不存在信号肽,为非分泌蛋白;IhCCR-1蛋白具有保守的KNWYCYGK催化位点,属于NADB-Rossmann超家族,相对分子量为37.61 kDa;Ih CCR-1与其他植物的CCR具有较高的亲缘关系。研究结果将为进一步研究七彩红竹木质素产生的分子机理和综合开发利用奠定基础。 Cinnamoyl-CoA reductase （CCR） is a key enzyme in the phenylpropanoid pathway, and plays a criticalrole in metabolic flow of lignin and anthocyanin. In this study, we cloned the full-length of eDNA of a novel CCRgene from Indosasa hispida ev. Rainbow with the method of reverse transcription PCR and gene-specific primers u-sing a semi-quantitative RT-PCR technique according to the transcriptome sequencing data. This novel gene wasnamed as IhCCR-1 （Accession Number： KP271440）. It has a length of 1 038 bp and encoded with a protein of345 amino acids. The IhCCR-1 protein is a stable acidic and non-secretory protein that has no signalpeptide. TheIhCCR-1 protein belonged to the NADB-Rossmann superfamily and contained the conserved KNWYCYGK motif.IhCCR-1 is closely related to the other plants. The results above would lay a theoretical basis for further explorationof molecular mechanism of lignin and for the comprehensive exploitation and utilization of lndosasa hispidaev. Rainbow.

关 键 词： 七彩红竹 木质素 花青素 肉桂酰辅酶 还原酶基因 克隆 基因分析

领　　域： [农业科学] [农业科学]