作　　者： ; ; ; ; ; ; (刘广芹）; (汪子豪）; (秦建如）;

机构地区： 华南农业大学兽医学院

出　　处： 《中国兽医杂志》 2015年第6期14-16,共3页

摘　　要： 为了探讨不同接种途径和日龄对SPF鸡感染J亚群禽白血病病毒(ALV-J)后感染模式的影响,本研究分别经11日龄鸡胚尿囊腔、1日龄口服、点眼滴鼻、腿部肌肉注射、颈部皮下注射、腹腔注射和7日龄腹腔注射途径接种了ALV-J CHN06株,于不同时间点(第1、2、4、6周),分别对各试验组的病毒血症、抗体及排毒进行了动态检测。结果显示,11日龄鸡胚尿囊腔组100%(15/15)感染模式为V+S+。1日龄口服组和点眼滴鼻组感染模式以V-S-为主。1日龄颈部皮下注射组、腿部肌肉注射组和腹腔注射组在第4、6周的感染模式均以V+S+为主。7日龄腹腔注射组在试验期内仅呈现一过性病毒血症,而且7日龄感染组在第6周的抗体阳性率比1日龄感染组高,差异显著(P<0.05)。本研究结果表明,胚胎感染易导致持续性病毒血症,注射途径比非注射途径更易诱导病毒血症和排毒。随着感染日龄的增加,病毒血症持续时间缩短,排毒时间推后,抗体生成水平升高;防止ALV-J早期感染有利于ALV-J的防控和净化。 To understand the effects of inoculation route and age on infection pattern of subgroup J avian leukosis virus （ALV-J）, SPF chickens were inoculated with ALV-J CHN06 via allantoic cavity on the eleventh day of embryonation （ 11 ED） and orally, ocu- lamasally, intramuscularly, subcutaneously, intra-abdominally on day 1 and intra-abdominally on day 7. At 1,2,4,6 w post inoculation, chickens were examined for ALV J viremia, ALV-J antibody and virus shedding. The results showed that the infection pattern of 11 ED group was predonminately V＋ S＋, while the oral and ocularnasal group were mainly V-S-.The infection patterns of the groups inoculated subcutaneously, intramuscularly and intra-abdominally on day 1 were mainly V＋S＋ .Transient viremla could be detected in the group of intra-abdommal inoculation on day 7.There was significant difference of seropositive rate at week 6 between the groups challenged on day 1 and day 7.The results showed that early prevention will be helpful in the eradication of ALV-J.

关 键 词： 亚群禽白血病病毒 接种途径 接种日龄 感染模式

领　　域： [农业科学] [农业科学] [农业科学]