作　　者： ; ; ; ; ; (谢木源）;

机构地区： 华南理工大学生物科学与工程学院

出　　处： 《华南理工大学学报（自然科学版）》 2014年第11期143-148,共6页

摘　　要： 为验证人骨髓间充质干细胞(h MSCs)的成骨定向分化,将成骨分化培养基培养作为成骨组,完全培养基培养作为对照组进行实验.VON KASSA染色结果显示:h MSCs具有良好的成骨分化能力,钙结节明显;碱性磷酸酶(ALP)酶活性检测结果显示:成骨组的ALP酶活性明显高于对照组(P<0.01),在12 d达到峰值,随后进入平台期.为验证h MSCs和聚乳酸-羟基乙酸/磷酸三钙(PLGA/TCP)的相容性,将PLGA/TCP上培养作为立体培养组、平面上培养作为平面对照组进行相关分析,扫描电镜结果显示细胞能在材料上粘附生长并进行成骨分化,四甲基偶氮唑盐(MTT)检测结果显示立体培养组的增殖率在5和7天时要明显高于平面对照组(P<0.01),ALP酶活性检测结果显示立体培养组的成骨分化效果在3、5、7天时要好于平面对照组(P<0.05),证明h MSCs和PLGA/TCP具有良好的生物相容性,两者具有结合生成组织工程骨的潜力. In order to verify the directional osteogenic differentiation of human mesenchymal stem cells（ hMSCs）,the corresponding experiments were conducted with the hMSCs cultured in the osteogenic differentiation medium as the osteogenic differentiation group and those in the complete medium as a control group. Von Kassa experiment shows that hMSCs has a good osteogenic differentiation ability with obvious calcium nodules. Alkaline phosphatase（ ALP） test demonstrates that the ALP activity of the osteogenic differentiation group is higher than that of the control group（ P 〈0. 01）,and reaches a peak in twelve days and then enters into a plateau phase. In order to verify the biocompatibility of hMSCs with poly lactide-co-glycolide（ PLGA） / TCP,the corresponding analyses are made with the hMSCs cultured in PLGA / TCP as the 3D culture group and those on plate as the 2D control group. SEM results show that adhesion,proliferation and osteogenic differentiation of hMSCs occur in PLGA / TCP. MTT test indicates that the cell proliferation rate of the 3D culture group in five and seven days（ P 〈0. 01） is obviously higher than those of the 2D control group. ALP test also demonstrates that the osteogenic differentiation of the 3D culture group in three,five and seven days（ P 〈0. 05） are higher than those of the 2D control group,which indicates that PLGA / TCP possesses good cellular biocompatibility with hMSCs and they have a potential to produce tissue engineering bones.

关 键 词： 骨组织工程 人骨髓间充质干细胞 聚乳酸 羟基乙酸 磷酸三钙 细胞增殖 成骨分化 生物相容性

领　　域： [生物学]