作　　者： ; (黄劲峥）;

机构地区： 肇庆学院化学化工学院

出　　处： 《食品与发酵工业》 2014年第4期233-237,共5页

摘　　要： 比较猴头菇多糖的几种提取方法。以猴头菇为原料,分别用热水提取法、超声波法、纤维素酶法、木瓜蛋白酶法、果胶酶法、复合酶法、超声复合酶法提取多糖,用苯酚-硫酸法测定猴头菇多糖含量,并对这几种方法的最佳提取条件和提取率进行了比较。结果表明:在最佳提取条件下,超声波法的多糖提取率为6.41%,热水提取法为5.80%,纤维素酶法为10.20%,木瓜蛋白酶法为9.77%,果胶酶法为7.94%,复合酶法为10.89%,超声复合酶法为15.59%,超声与复合酶法的协同作用可使多糖提取率大为提高。 Several extraction methods of polysaccharide from Hericium erinaceus were compared. Polysaccharide was extracted from Hericium erinaceus by the following methods： hot water extraction, ultrasonic wave extraction, cellulose extraction, papain extraction, pectinase extraction, compound enzyme extraction and ultrasonic wave combined with compound enzyme extraction. The phenol-sulfuric acid method was applied to measure the content of polysaccharide. The optimized conditions and the extraction ratios were investigated and compared. The results indicated that under the optimized conditions, the extraction ratios of Hericium erinaceus polysaccharide were 6.41% in ultrasonic process, 5.80% in hot water process, 10.20% in cellulose enzyme process, 9.77% in papain process, 7.94% in pectinase process, 10.89% in multi-enzymatic process and 15.59% in ultrasonic combined with multi-enzymatic process. The polysaccharide yield was improved considerably with ultrasonic wave and compound enzyme method.

关 键 词： 猴头菇 多糖 热水提取 超声 酶法 超声酶法 比较

领　　域： [轻工技术与工程] [轻工技术与工程]