作　　者： ; ; ;

机构地区： 信阳农林学院

出　　处： 《中国食品添加剂》 2014年第8期182-186,共5页

摘　　要： 包埋法是固定化技术中应用最为广泛的一种方法,其中的凝胶包埋法以其原料简单、容易操作的特点被广泛采用。在对包埋法固定肠膜明串珠菌的材料选择上,选取了四种海藻酸盐凝胶——海藻酸钙凝胶、海藻酸铝凝胶、海藻酸锰凝胶和经戊二醛处理的海藻酸钙凝胶,设计实验对四种材料的机械性能、吸收性能、释放性能和产糖量进行对比,结果表明:四种材料的机械强度大小排序为:海藻酸铝﹥海藻酸锰﹥戊二醛处理海藻酸钙﹥海藻酸钙;吸收性能大小排序为:戊二醛处理海藻酸钙﹥海藻酸锰﹥海藻酸钙﹥海藻酸铝;释放性能大小为:海藻酸锰﹥海藻酸钙﹥海藻酸铝﹥戊二醛处理海藻酸钙;产量高低排序为:海藻酸钙﹥海藻酸锰﹥海藻酸铝﹥戊二醛处理海藻酸钙。通过对比,综合考虑多方面因素,最终选取海藻酸钙凝胶作为固定化肠膜明串珠菌的材料。 Embedding is the most widely used in immobilization technology, and gel embedding method is widely used for its simple raw materials and easy operation. In the selection of materials for immobilizing Leuconostoc mesenteroides by embedding, we selected four alginate gels： calcium alginate, aluminum alginate, manganese alginate and calcium alginate with glutaraldehyde treatment. Test results of the mechanical property, absorption property, release property and sugar yield of the four materials showed that the order of mechanical property was ： aluminum alginate 〉 manganese alginate 〉 calcium alginate with glutaraldehyde - treated 〉 calcium alginate; the order of absorption property was： calcium alginate with glutaraldehyde- treatment 〉 manganese alginate 〉 calcium alginate 〉 aluminum alginate; the order of release property was： manganese alginate 〉 calcium alginate 〉 aluminum alginate 〉 calcium alginate with gl- utaraldehyde - treatment; the order of sugar yield was ： calcium alginate 〉 manganese alginate 〉 aluminum alginate 〉 calcium alginate with glutaraldehyde - treatment. By contrast and considering various factors, we selected calcium alginate as the immobilizing material of Leuconostoc mesenteroides.

关 键 词： 包埋法 固定化 肠膜明串珠菌 海藻酸钙

领　　域： [生物学]