作　　者： ; ; ; ; (林光月）;

机构地区： 广东省农业科学院

出　　处： 《食品与生物技术学报》 2014年第7期721-726,共6页

摘　　要： 150只健康昆明小鼠随机分为3批(5组/批),分别用于负重游泳实验和生化指标的检测。5组动物依次分为阴性对照组(control group,CG)、阳性对照组(Yikang Capsule,YKC)、玛咖低、中、高剂量组(L-MACA,M-MACA,H-MACA)。阳性对照组给予益康胶囊(剂量0.30 g/(kg·d)),玛咖组分别灌胃给予MACA 0.03、0.30、1.00 g/(kg·d),持续30 d。测定各组小鼠负重游泳时间、比色法测定血清尿素氮(BUN)、血乳酸(LAC)、血红蛋白(Hgb)、乳酸脱氢酶(LDH)和肝糖原(LG)。比色法或HPLC法对玛咖中抗疲劳相关活性组分氨基酸、多糖、果糖和芥子油苷等进行定量检测。结果表明:玛咖含有丰富的氨基酸(158.10 mg/g)、总黄酮(20.83 mg/g)、多糖(37.16 mg/g)、果糖(31.27 mg/g)、总生物碱(4.22 mg/g)、芥子油苷(1.08 mg/g)等成分。抗疲劳动物试验结果表明,与阴性对照组相比,MACA高、中、低剂量组均能显著延长健康小鼠负重游泳时间(P≤0.05);MACA低剂量组能显著降低血乳酸含量(P≤0.05);MACA中剂量组能显著降低血清尿素氮的含量,显著增加血红蛋白和肝糖原的含量P≤0.05);MACA高剂量组能显著增加血红蛋白和肝糖原的含量(P≤0.05)。玛咖富含抗疲劳相关的多种活性组分,对健康小鼠具有显著的抗疲劳功能,是开发抗疲劳功能食品的理想原料。 150 healthy Kunming mice were randomly divided into three batches （5 groups each batch） for loaded swimming test and biochemical analysis.We divided Maca into low,medium and high dose groups （0.03 g/kg.bw.d,0.30 g/kg.bw.d,1.00 g/kg.bw.d）,and the control group （saline）,positive control group （YiKang capsules for 0.30 g/kg.bw.d） respectively.Mice were administered ig daily for 30 d,then the loaded-swimming time,blood urea nitrogen,blood lactate,hemoglobin,lactate dehydrogenase and hepatic glycogen of them were measured.In addition,active ingredients related to anti-fatigue in Maca such as amino acids,polysaccharides,fructose and glucosinolates were quantitative detected through colorimetry or HPLC.Results show that,Maca is rich in amino acids （158.10 mg/g）,total flavonoids （20.83 mg/g）,polysaccharides （37.16 mg/g）,fructose （31.27 mg/g）,total alkaloids （4.22 mg/g）,mustard oil glycosides （1.08 mg/g） and other ingredients.Animal testing results show that,the high,middle,low dose groups could all significantly prolong the time of loading swimming,increase the hemoglobin,lactate dehydrogenase and hepatic glycogen,decrease blood urea nitrogen and blood lactate in mice compared with negative control.Maca is rich in a variety of active ingredients related to anti-fatigue,and it has significant anti-fatigue function in healthy mice,may be ideal raw material for anti-fatigue functional foods.

关 键 词： 玛咖 有效成分 抗疲劳活性

领　　域： [农业科学] [农业科学]