作　　者： ; ; ; ; ; ; ; ; (蒲云丹）; (徐敬钧）;

机构地区： 四川农业大学动物医学院鱼病研究中心

出　　处： 《中国预防兽医学报》 2014年第8期636-640,共5页

摘　　要： 为鉴定一起齐口裂腹鱼突发传染病的病原,本研究从患病濒死鱼中分离到两株致病菌并对其进行常规理化特性、分子生物学、病理学和致病性试验以及药敏性检测。结果显示,两分离株理化特性与迟钝爱德华菌(E.tarda)特征相符,与GenBank中E.tarda的16S rDNA、gyrB基因、fimA毒力基因和gadB毒力基因的序列同源性均达99%以上,在系统发育树中与E.tarda聚为一族,毒力基因双重PCR扩增出443 bp和584 bp两条特异性片段,综合确认分离株为致病性E.tarda,将其命名为Et-1和Et-2。人工感染鱼出现与自然发病相似症状,并能从发病鱼组织中再分离到相同病原菌。分离菌株对20种抗生素中氟苯尼考、左氧氟沙星等10种药物敏感。 Two bacteria were isolated from diseased Schizothorax prenanti in a farm. The bacteria were identified to be Edwarsdsiella tarda based on physiological and biochemical tests, amplifications of 16S rDNA and gyrB gene. In addition, the 443 bp and 584 bp DNA fragments of the virulence genes of fimA and gadB were amplified from both isolates of E. tarda by PCR. The main histopathologic changes were found in liver, kidney, intestine, brain, gill and muscle. Furthermore, the artificially infected fish showed the same clinical signs and pathologic changes, and the same bacteria were re-isolated from the diseased fish. Antibiotic sensitivity assays showed that among 20 antibiotics tested 10 were sensitive, and 7 were resistant.

关 键 词： 迟钝爱德华菌 齐口裂腹鱼 病理损伤

领　　域： [农业科学] [农业科学] [农业科学]