作　　者： ; ; ;

机构地区： 华南农业大学动物科学学院动物营养与饲料科学系

出　　处： 《华南农业大学学报》 2001年第4期71-74,共4页

摘　　要： 试验选用 30、6 0、90日龄粤黄鸡各 30只 ,捕杀后取脾脏 ,除去外周结缔组织及被膜 ,用匀浆透析法提取脾脏小分子活性物质 ,经SephadexG - 2 5凝胶柱层析系统进行柱层析 ,采用氨基酸自动分析仪分析各组分的氨基酸组成 ,并通过紫外光吸收光谱来进行扫描光谱分析 结果表明 :鸡脾脏小分子活性物质提取液经SephadexG - 2 5柱层析可分离到 3个峰 ,各峰理化性质存在差异 ;不同日龄鸡脾脏小分子活性物质的同一组分所含氨基酸种类基本相同 。 Thirty Yuehuang broilers aged 30, 60,90 days respectively were slaughtered and the decapsulated spleen collected. The spleen micromolecule active mass was extracted by homogenation dialysis.After sterile filtration,the extract was dispensed in small batches and stored at -20 ℃.The proximate analysis was conducted with Sephadex G-25 size exclusion chromatography system, automatic amino acid analyzer and untraviolet spectrophotometer.The results showed that three fractions were isolated from the extract of chicken spleen micromolecule active mass by Sephadex G-25 size exclusion chromatography,the physiochemical properties among the fractions were different. The amino acid species in the extracts of chicken spleen micromolecule active mass at different ages of birds were similar, while the composition and the amount of amino acids among the fractions were different.

关 键 词： 鸡 脾脏小分子活性物质 柱层析 转移因子 组分分析 氨基酸

领　　域： [农业科学] [农业科学] [农业科学] [农业科学] [农业科学]