作　　者： ; ;

机构地区： 华南农业大学兽医学院

出　　处： 《中国兽医杂志》 2001年第12期3-7,共5页

摘　　要： 用聚合酶链反应 (PCR)技术 ,制备了广东禽流感无致病力分离株 A/goose/China/2 4/96 (H7N3 )核蛋白基因片段(NPc)的地高辛标记的 c DNA探针。建立并优化了检测禽流感病毒核酸的探针杂交法 ,探针杂交法能鉴别出非免疫鸡胚和SPF鸡胚尿囊液中的病毒 ,攻毒后第 3天的 SPF和非免疫鸡泄殖腔拭子中 AIV的最大检出率为 1/10 ,对临床样品中的 AIV的最大检出率为 1/7,而直接 HA和 HI法及 AGP试验检不出临床样品的 AIV。该探针具有较好的特异性和敏感性 ,为从分子水平探讨 AIV的发病机理、临床早期快速诊断提供了新的研究手段。 A cDNA probe of the partial genome segment of avian influenza virus (AIV) was labelled with DIG by the technique of PCR. It was used for detection of virus RNA in AIV infected chicken embryo allantoic fluids clinical specimens from various avian. The positive rate of cloacal swab from non immunized chickens and SPF chickens experimentioly infected with AIV were 10%(1/10) by dot hybridization on the third day p.i.. It was 14.3%(1/7) for clinical samples. However, direct HA, HI AGP test could not detect AIV in these clinical smples. This showed that the methods of dot hybridization established were more sensitive, rapid and accurate than conventional serologic test. It can be applied for study on the pathogenesis in molecular level.

关 键 词： 地高辛标记 探针 禽流感病毒 核酸检测 禽流感

领　　域： [农业科学] [农业科学] [农业科学] [农业科学] [农业科学] [农业科学]