作　　者： ; ; ; ; ; ; ;

机构地区： 广东省农业科学院

出　　处： 《农业生物技术学报》 2001年第1期33-35,共3页

摘　　要： 甜菜夜蛾（Ｓｐｏｄｏｐｔｅｒａ　ｅｘｉｇｕａ）核型多角体病毒（ＳｅＮＰＶ）克隆株 Ｇ１、 Ｇ３、 Ｇ４及 ＳｅＮＰＶ１感染 ＳｐＩｍ、 ＢｍＮ４和Ｌｄ６５２ｙ细胞后，均能引起细胞凋亡现象。 ＳｐＩｍ细胞感染９６ｈ后，大部分细胞死亡，形成葡萄状小泡，但能在个别细胞中形成病毒多角体；ＢｍＮ４细胞感染４８ｈ后，细胞内颗粒状物质增多，７２ｈ后全部死亡，脱壁上浮；Ｌｄ６５２ｙ细胞被 Ｇ３及 ＳｅＮＰＶｌ感染７２ｈ后解体成颗粒状，而Ｇ１、 Ｇ４只能令部分细胞死亡。 Ｗｅｓｔｒｅｒｅｎ　Ｂｌｏｔ及Ｓｌｏｔ　Ｂｌｏｔ分析表明：ＳｅＮＰＶ克隆株能在ＳｐＩｍ及ＢｍＮ４细胞中复制病毒ＤＮＡ，且能在ＳｐＩｍ细胞中形成多角体蛋白，但不能在Ｌｄ６５２ｙ细胞中复制ＤＮＡ。 The phenomenon of programmed cell death was observed in the non host cell lines Ld652y (Lymantria dispar), BmN4 Bombyx mori) and SpIm (Sphilosoma imparilis) by infecting with the clones of G1, G3, G4 and SeNPV of nuclear polyhedrons virus from army worm (Spodoptera exigua, Se). After infected with SeNPV, the Ld652y cell died and some granular were found in the cell; the BmN4 cell left the wall and float in the medium; the SpIm cell developed some grape-shape vacuoles and the polyhedrin bodies were found in few cells. The result of Westreren Blot and Slot Blot showed that the SeNPV DNA can replicate in both SpIm and BmN4 cell, but the polyhedrin protein developed in SpIm cell only. The pathologic characterizes of SeNPV infection were different between Ld652y and SpIm cell lines.

关 键 词： 克隆株 非宿主细胞 病理效应 甜菜认蛾核型多角体病毒

领　　域： [农业科学] [农业科学] [农业科学] [农业科学]