作　　者： ; ; ; ; ; ;

机构地区： 遵义医学院珠海校区

出　　处： 《遵义医学院学报》 2014年第3期300-303,共4页

摘　　要： 目的探讨饲料中Cu2+在黑水虻体内迁移规律及对其发育影响。方法以添加不同浓度Cu2+的人工饲料饲喂黑水虻幼虫,利用等离子体原子发射光谱仪测定各发育阶段虫体的Cu2+含量;利用昆虫毒理学方法对其发育影响进行研究。结果黑水虻各发育阶段虫体Cu2+含量均随处理浓度增加而增加,1200 mg·kg-1处理浓度时Cu2+含量最高;Cu2+可随黑水虻的发育而迁移,高浓度胁迫下迁移规律为:蛹>6龄幼虫>成虫。Cu2+胁迫对黑水虻幼虫存活率、化蛹率、成虫寿命及雌雄性比均无显著影响,但高浓度处理可显著降低蛹的羽化率并延长幼虫及蛹发育所需时间。结论 Cu2+可在黑水虻体内进行迁移,高浓度胁迫下迁移规律为:蛹>6龄幼虫>成虫;仅一个世代Cu2+胁迫对黑水虻幼虫存活率、化蛹率、成虫寿命及雌雄性比影响较小,主要降低蛹的羽化率及延长幼虫及蛹发育所需时间。 Objective To investigate the migration disciplines of Cu2 ＋in black soldier flies and its effects on the development.Methods Black soldier fly larvae were fed with different concentrations of Cu2 ＋.Cu2 ＋concentrations in insect of each developmental stages were measured by plasma atomic emission spectrometer.Effects of Cu2 ＋on the development were assessed by insect toxicology method.Results Cu2 ＋concentrationsin insect of each developmental stages were increased with the elevated concentrations of Cu2 ＋treatments,in which Cu2 ＋（1200mg.kg-1） treatment lead to the highest Cu2 ＋concentrations in insect,Cu2 ＋migrated with the development of black soldier flies and the migration discipline after high Cu2 ＋concentrations treatment was in pupation 〉 six instar larva 〉 adult.There was no significant difference of larva survival rate,Pupation rate,adult longevity and sex ratio in the stress of Cu2 ＋on black soldier flies,whereas high Cu2 ＋concentration treatment significantly reduced the eclosion rate and prolonged the development of larva and pupation.Conclusion Cu2 ＋migrates in black soldier flies and its migration disciplines after high Cu2 ＋concentrations treatment was in pupation 〉 six instar larva 〉 adult.Only one generation of Cu2 ＋stress has no significant differences in larva survival rate,pupating rate,adult longevity and sex ratio and mainly reduces eclosion rate and prolongs the development of larva and pupation.

关 键 词： 铜 胁迫 黑水虻 累积 发育

领　　域： [生物学]