作　　者： ; ; ; ; ; ; ;

机构地区： 深圳职业技术学院应用化学与生物技术学院

出　　处： 《生物技术通报》 2014年第5期190-196,共7页

摘　　要： 旨在研究鲎素Ⅰ对普通变形杆菌的抑杀作用机制。利用鲎素Ⅰ对普通变形杆菌的抑杀浓度、壁膜通透性和电荷性、分泌蛋白酶活力的影响及其在培养基中的稳定性进行研究。结果显示,鲎素Ⅰ对普通变形杆菌的MIC和MBC值分别为40-80和80μg/mL,它也能增加普通变形杆菌的壁膜通透性及其所带的正电荷。在鲎素Ⅰ浓度≤40μg/mL时,普通变形杆菌分泌的蛋白酶活力有所增加,而≥60μg/mL时,则呈下降趋势。在普通变形杆菌培养环境中的鲎素Ⅰ含量会降低。结果证明,鲎素Ⅰ能对普通变形杆菌产生抑杀作用,其抑菌机制与破坏细菌壁膜的通透性有关。 It was to investigate the effect mechanism of tachyplesin I on Proteus vulgaris. The study on the inhibition concentration of tachyplesin I, the plasma membrane permeability and electric charge of Proteus vulgaris, the activity of proteases were secreted by Proteus vulgaris and the stability of tachyplesin I in the nutrient broth medium where cultured Proteus vulgaris. Results indicated that the MIC and MBC were 40-80 and 80 μg/mL, respectively. The membrane permeability and electric charge of Proteus vulgaris would be increased with the concentration of tachyplesin I increasing. The proteinase activity which secreted by Proteus vulgaris would be increased when the concentration of tachyplesin I less than 40 μg/mL, but it presented downward trend when more than 60 μg/mL. The concentration of tachyplesin I in the medium that trained the bacterial would reduce. Tachyplesin I could inhibit growth of the Proteus vulgaris, the effect mechanism related to damage the membrane permeability of them.

关 键 词： 鲎素 普通变形杆菌 抑菌作用 蛋白酶活

领　　域： [农业科学] [农业科学] [农业科学]