作　　者： ; ; ; (王继楠）;

机构地区： 东北农业大学

出　　处： 《营养学报》 2014年第2期164-167,共4页

摘　　要： 目的探讨十种常见抗生素对于人体肠道菌群体外转化黄豆苷元为雌马酚的影响,确定抗生素对雌马酚合成的影响。方法实验利用雌马酚产生者的肠道菌群进行体外代谢,收取雌马酚产生者的新鲜粪样适量,在10 ml BHI肉汤培养基中厌氧罐内37℃恒温培养24 h,再到10 ml BHI-黄豆苷元肉汤培养基中37℃厌氧培养96 h。将不同浓度的抗生素加入到BHI-黄豆苷元培养基后,加入处理好的粪样,37℃厌氧培养24h,收集培养液待HPLC检测。结果当加入的抗生素浓度为5μg/ml时,样品中盐酸环丙沙星的抑制作用最小,其次为硫酸粘菌素、青霉素与氯霉素;头孢曲松抑制作用最强,低浓度下已没有雌马酚检出;当抗生素浓度升高后,除土霉素、盐酸多西环素以及头孢曲松外,其余样品在抗生素作用下都可继续产出雌马酚。结论不同种类抗生素对人体肠道菌群中黄豆苷元转化为雌马酚的抑制作用不同,盐酸环丙沙星对雌马酚产出抑制作用最小,头孢曲松、盐酸多西环素以及土霉素基本完全抑制。 Objective To explore the effects of antibiotics on equol production. Methods Fresh fecal samples containing equol were cutured in 10ml BHI broth in 37℃ anaerobic tank for 24 h, and then cultured in 10ml BHI-daidzein broth for 96h. Different concentrations of antibiotics were added to the BHI-daidzein medium, and cultured for 24 h, The cultures solution was collected for HPLC detection. Results When the concentration of antibiotics added was 5 p^g/ml, inhibitory effect of ciprofloxacin hydrochloride in the samples was the weakest followed by colistin sulfate, penicillin and chloramphenicol, and ceftriaxone was the strongest. In low concentration, no equol was detected. When the antibiotic concentration increased, equol contistillnuously was still produced except soil, clindamycin, doxycycline and ceftriaxone. Conclusion Inhibitory effects of different antibiotics on equol-production by intestinal microflora are different, Ciprofloxacin hydrochloride acts the weakest, while ceftriaxone, doxycycline and oxytetracycline are the strongest.

关 键 词： 雌马酚 抗生素 肠道菌群

领　　域： [轻工技术与工程] [轻工技术与工程]