作　　者： ; ; ; ; ;

机构地区： 四川农业大学动物医学院

出　　处： 《湖南农业大学学报（自然科学版）》 2014年第2期188-191,共4页

摘　　要： 选择28日龄"杜×长×大"断奶仔猪90头,随机分为3组:对照组饲喂基础日粮;低剂量组饲喂基础日粮+0.1%蛹虫草菌发酵五味子药渣菌质;高剂量组饲喂基础日粮+0.2%蛹虫草菌发酵五味子药渣菌质。每组3个重复,每个重复10头。饲喂14 d后,从每个重复中随机选取1头仔猪,前腔静脉采血,测定血液生理成分,外周血淋巴细胞亚群和增殖活性,以及血清中白细胞介素–2(IL–2)和γ–干扰素(IFN–γ)的含量。结果:与对照组相比,低剂量组和高剂量组仔猪的白细胞(WBC)、淋巴细胞(LYM)、平均血红蛋白浓度(MCHC)、血小板(PLT)、平均血小板体积(MPV)和血小板分布宽度(PDWcv)均无显著性差异(P<0.05);高剂量组红细胞(RBC)和血红蛋白(HGB)含量显著增加(P<0.05);低剂量和高剂量组CD4+T细胞与CD8+T细胞比值、淋巴细胞增殖指数均极显著增加(P<0.01);高剂量组血清IFN–γ含量显著提高(P<0.05或P<0.01),各组猪的血清IL–2含量的差异均不显著(P>0.05)。以上结果表明,五味子药渣发酵菌质能优化仔猪血液的生理指标,提高仔猪的免疫功能。 Ninety crossbred weaned piglets ＂Duroc × Landrace x Yorkshire＂ aged 28 days were randomly allocated into 3 groups （3 replicates for each group and 10 pigs for each replicate）. Pigs in the control group were fed with basic diet, in low-dose group with basic diet plus 0.1% Cordyceps militaris fermented Schisandra chinensis residue and in high-dose group with basic diet plus 0.2% Cordyceps militaris fermented Schisandra chinensis residue for 14 days. Then one pig from each replicate was selected for blood collection by anterior vena cava venipuncture for analysis of the routine blood parameters, peripheral T lymphocyte subgroups and their proliferative activities, as well as the IL-2, IFN-γ contents. Results showed there was no difference in white blood cells （WBC）, lymphocyte （LYM）, mean erythrocyte haemoglobin concentration （MCHC）, platelet count （PLT）, mean platelet volume （MPV） and platelet distribution width （PDWcv） （P〉0.05） between the control group and the low- and high-dose groups. Compared to the control, the contents of RBC and HGB in high-dose group were significantly increased （P〈0.05）, the CD4＋/CD8＋ ratio and proliferation function of peripheral blood T cell in low- and high-dose groups were extremely significantly increased （P〈0.01）, the serum IFN-γ contents in high-dose group were also increased （P〈0.05 or P〈0.01）, but there was no significant difference in serum IL-2 contents of piglets among groups （P〉0.05）. It was concluded that dietary supplied with Cordyceps militaris fermented Schisandra chinensis residue improves the immunologic function and optimizes blood physiology parameters of piglets.

关 键 词： 仔猪 五味子药渣 发酵 蛹虫草菌 血液指标 免疫活性

领　　域： [农业科学] [农业科学] [农业科学] [农业科学] [农业科学] [农业科学]