作　　者： ; ; ; ;

机构地区： 山东农业大学农学院

出　　处： 《核农学报》 2014年第2期193-199,共7页

摘　　要： 对来源于陕西商洛、河南内乡、四川中江、山东泰安4个产地共80个丹参株系种质资源进行EST-SSR分析,旨在探讨栽培丹参资源的多样性水平和产地间的遗传分化程度。结果表明,栽培丹参Nei基因多样度为0.2245,揭示出具有丰富的遗传多样性,且各产地丹参遗传分化程度低,遗传变异主要存在于产地内部;4个产地相比较,四川产地明显较低。UPGMA聚类分析结果表明,山东产丹参是相对独立的群体,但4个产地来源丹参的遗传距离与地理距离相关性未达到显著水平。栽培丹参具有较高的多样性以及产地间遗传分化程度较低,其异花授粉习性和产地内采用种子有性繁殖方式以及稍加人为选择的生产方法可能是其栽培丹参多样性高和产地间分化低的主要原因。 The purpose of the study was to determine the genetic diversity of Salvia Miltiorrhiza Bge from four different cultivated habitats with 10 EST-SSR primers. The result showed that a high level of genetic diversity at the species level was detected （H = O. 2245 ） and the low degree of genetic differentiation. The genetic variation mainly consisted in population based on Nei＇ s coefficient of differentiation （GST）. Genetic diversity of Sichuan in four habitats was significantly lower. The UPGMA cluster analysis indicated that Shandong habitat was the farthest with the other three populations and was relative independent habitats. The genetic relationships among habitats have low correlation with their geographical distribution. Our results suggested that outcrossing breeding system, planting seeds without deliberate selection might be those reasons result in the high genetic diversity at the species level and the low genetic differentiation among habitats.

关 键 词： 丹参 遗传多样性

领　　域： [农业科学] [农业科学]