作　　者： ; ; (毕佳捷）;

机构地区： 大连工业大学

出　　处： 《大连工业大学学报》 2013年第6期432-436,共5页

摘　　要： 建立了用离子色谱测定造纸原料中常见的7种单糖和2种糖醛酸含量的方法。造纸原料经过酸水解，稀释过滤后直接进样上机分析。结果表明，在以CarboPac-TM PA20阴离子交换柱为分离柱，用2mmol／LNaOH溶液等梯度淋洗20rain，而后用NaAc（50～200mmol／L）和2retool／L NaOH溶液梯度淋洗10min，淋洗液体积流量为0．5mL／min的分离条件下，7种单糖和2种糖醛酸分离情况最佳。标准混合糖6次进样，各单糖浓度的变异系数为0．73％～1．64％。用所建立的方法分析了桉木和玉米秸秆中纤维素和半纤维素酸水解后产生的单糖种类和含量。结果表明，2种原料中最主要的单糖是葡萄糖，其次是木糖；在桉木中没有检测出果糖和半乳糖醛酸，在玉米秸秆中没有检测出果糖。并用测得的结果计算了这2种原料中综纤维素和聚戊糖的含量，与用国家标准方法测出的结果相当。 Seven monosaccharides and two uronic acids were determined by ion chromatography after paper making material was hydrolyze. Seven monosaccharides and two uronic acids were optimally separated with Dionex CarboPacTM PA20 anion exchange column eluted isocratically with 2 mmol/L NaOH for the first 20 minutes and eluted gradiently with NaAc （50-200 mmol/L） and 2 mmol/L NaOH for the next 10 minutes at 0.5 mL/min flow rate. RSD of each monosaccharide were 0.73%-1.64%. When applying for determining the kind and content of monosaccharides and uronic acids in acidic hydrolysate of cellulose and hemicellulose in eucalyptus and corn stover, the results showed the major glucose followed by xylose. Fructose and galacturonic acid were not detected in eucalyptus and fructose was not detected in corn stover. Holocellulose content and pentosan content in those two materials were calculated to be equivalent to the results obtained by national standard methods.

关 键 词： 离子色谱 造纸原料 单糖 糖醛酸

领　　域： [轻工技术与工程]