作　　者： ; ; ;

机构地区： 南京体育学院

出　　处： 《山东体育科技》 2014年第1期67-71,共5页

摘　　要： 目的:探讨运动与肉碱补充对肝脏胆固醇代谢的影响。方法:通过饲喂高脂日粮将C57BL/6小鼠建立营养性肥胖模型。随机分为4组:对照组、运动组、左旋肉碱组及运动结合左旋肉碱组。5周后采集血液、肝脏组织,检测血清和肝脏中的胆固醇水平及胆固醇代谢关键基因3-羟-3-甲基戊二酸单酰CoA还原酶(HMGCR)、肝脏X受体(LXR)和固醇调节元件结合蛋白2(SREBP2)的基因表达。结果:无论是运动还是左旋肉碱补充均使肥胖小鼠体重显著降低,而运动跟肉碱同时处理对体重的影响更大。运动显著增高血清中总胆固醇(Tch)的水平(P<0.05),并且显著增高低密度脂蛋白(LDL)的水平(P<0.05)。运动与肉碱同时处理显著增高血清高密度脂蛋白(HDL)的水平(P<0.05)。运动及运动与肉碱同时处理均显著上调胆固醇合成的限速酶——HMGCR的蛋白表达(P<0.05),并且与单纯肉碱组相比,运动对HMGCR的蛋白表达有上调的趋势(P=0.073)。运动与肉碱同时处理显著上调LXR和SREBP2的基因表达(P<0.05)。结论:运动结合左旋肉碱补充通过刺激HMGCR、LXR和SREBP2的表达上调HDL的水平,运动主要通过HMGCR调节胆固醇及脂蛋白的代谢,而单纯左旋肉碱补充对胆固醇代谢没有显著影响。 Objective：To investigate the effects of exercise and L- carnitine supplementation on cholesterol metabolism in the liver. Methods：To establish nutritional obesity model by fedding high- fat diets to C57BL/6 mice. Obesity mice were randomly divided into 4 groups, tile control group, the exercise group, fedding L- carnitine group and exercise combined L- carnitine supplementation group. 5 weeks later, miee were killed, and blood, liver tissue were collected. Lipids in serum and cholesterol metabolism key enzymes 3 - hydroxy - 3 - methyl - glutao＇l CoA reductase （ HMGCR ）, liver X receptor （ LXR ） and sterol regulatory element binding protein 2 （SREBP2） in liver were tested. Results ：The body weights were significantly low in 3 experiment groups （ P 〈 0. 05 ）, especially in exercise combined L - carnitine supplementation group. Total cholesterol （Tch） and low density lipoprotein （LDL） in serum were significantly increased in exercise group （P 〈 O. 05）. High density lipoprotein （HDL） in serum was significantly increased in exercise combined L- carnitine supplementation group（ P 〈 O. 05）. The expression of cholesterol synthesis rate - limiting enzyme - HMGCR was significantly up- regulated in exercise group and in exercise combined L- carnitine supplementation group （P 〈 O. 05 ） , and compared with fedding L - carnitine group, there was upregulated tendency of HMGCR protein expression （P = O. 073 ） in exercise group. The expression of LXR and SREBP2 was upregulated in exercise combined L - carnitine supplementation group （P 〈 O. 05 ）. Conclusion： Exercise combined with L - carnitine supplemental＇. may up - regulated the concentration of HDL by stimulation expression of HMGCR, LXR and SREBP2. Exercise regulates the metabolism of cholesterol and lipoprotein mainly through LXR and SREBP2, while L - carnitine supplementation major influence HMGCR to regulate cholesterol metabolism.

关 键 词： 运动 左旋肉碱 肥胖 肝脏 胆固醇

领　　域： [文化科学] [文化科学]