作　　者： ; ; (等）;

机构地区： 中山大学化学与化学工程学院

出　　处： 《环境科学学报》 2001年第1期13-17,共5页

摘　　要： 从处理石化厂废水的活性污泥中分离出一株邻苯二甲酸酯降解菌FS1(PseudomonasfluorescensFS1) ,测定了P .fluo rescensFS1的邻苯二甲酸酯降解酶对邻苯二甲酸二 (2 乙基己基 )酯 (DEHP)的降解特性 .P .fluorescensFS1细胞中的颗粒部分、溶液部分对DEHP都具有降解作用 ,初步认为P .fluorescensFS1降解酶属胞内酶 .P .fluorescensFS1降解酶对DEHP的最适酸度为 pH 6 .5— 8.0 ,温度为 2 5— 35℃ ,米氏常数Km为 190 .48nmol/mL .在好氧条件下 ,DEHP与P .fluorescensFS1降解酯酶作用 ,形成邻苯二甲酸单酯和邻苯二甲酸后 ,可进一步降解成苯甲酸、对羟基苯甲酸 ,最终转化为CO2 和H2 Degradative characteristics of di(2 ethylhexyl)phthalate were determined by the crude enzyme extracted from Pseudomonas fluorescens FS1 which was isolateed from the activated sludge at a petrochemical factory. The results showed that the PAEs\|degrading enzyme, mainly locatized in soluble part of cytoplasm, is an intracllular enzyme. The optium temperature and pH of the PAEs\|degrading enzyme activity to di(2\|ethylhexyl)phthalate are 20—35℃ and 6.5—8.0, respectively .The Michaelis mentn's constant ( K m) of di(2\|ethyl hexyl)phthalate enzymatic degradation are about 190.48?nmol/mL at 30℃. The enzymatic metabolism of di((2 ethyl hexyl)) phthalate under aerobic conditions begins by PAEs degrading enzyme to monoester, further enzymatic degradation of monoester proceeds via phthalic acid by benzoate, 4 hydroxy benzoic acid, and then to CO 2 and H 2O.

关 键 词： 邻苯二甲酸酯 乙基已基 微生物 酶促降解 生物降解 有机污染物

领　　域： [环境科学与工程]