作　　者： ; ; ; ; (葛延松）; (曹嫦妤）;

机构地区： 东北农业大学动物医学学院

出　　处： 《浙江大学学报（农业与生命科学版）》 2014年第1期9-15,共7页

摘　　要： 应用生物软件分析鸡和其他11种脊椎动物硒蛋白 T （ selenoprotein T ,SelT ）的硒代半胱氨酸插入序列（ selenocysteine insertion sequence , SECIS）元件、SelT核苷酸和氨基酸序列的同源性,并分析鸡SelT 的结构及功能；采用实时荧光定量PCR（ fluorescent quantitative real-time PCR , fqRT-PCR）方法检测SelT基因在35日龄鸡体内30种组织中的表达谱.结果显示：脊椎动物 SelT的SECIS元件均属于Ⅱ型结构；鸡 SelT核苷酸序列与其他11种脊椎动物的同源性在48.0％～85.1％之间,而氨基酸序列与非洲爪蟾、斑马鱼的同源性低于90.0％,与其他9种动物的同源性在90.6％～94.9％之间；鸡 SelT 属于跨膜蛋白,存在信号肽,属于 RDx 家族,酶活性分类为EC 2.5.1.18,具有氧化还原功能,且存在Ca2＋结合位点.SelT在鸡各组织中广泛表达,在睾丸中含量极其丰富,提示鸡SelT在雄性生殖系统中可能发挥功能. Selenium （ Se） was an important , biologically necessary trace element , which played an important biological roles in avian growth , reproduction , immune function and disease resistance . The biological significance of Se was attributed to its occurrence in selenoproteins in the form of selenocysteine （ Sec） with a 21 amino acid genetic code . Se uses the stop codon UGA as the coding codon encoding Sec . Selenoproteins involved in many life processes such as antioxidant defense , cell signal transduction , metabolic pathway , development , immune function , and hormone regulates , and it was related to the occurrence and mechanism of many diseases . Selenoprotein T （SelT） was originally identified through silico studies ,cloned and expressed in a mammalian cell line ,confirmed as a selenoprotein . Previous studies have proved that SelT mainly occurred in Golgi apparatus and endoplasmic reticulum , with important biological function . SelT can act on the Ca2＋ homeostasis , involved in neuroendocrine secretion . SelT has also been showed in alter cell adhesion . Up to now , 25 selenoprotein-encoding genes were identified in birds , however , only about half have been functionally characterized , most of which were involved in redox reactions . SelT was a Se-containing protein whose cellular function has not been characterized . Research on the structure and function of SelT was still in its infancy , but reports in mammals about SelT and the information about avian SelT were not clear . The purpose of this study is to explore the selenocysteine insertion sequence （ SECIS） element , structures and functions of chicken SelT and the expression profiles of SelT in chicken tissues . The chicken SelT sequence was used in the experiment . The SECIS elements of 12 vertebrates were analyzed by the SECISearch 2.19 . The homologies of the molecule nucleotide and amino acid sequences on vertebrates were analyzed by DNAStar . The structure and functions of chicken SelT were predicted with the bioinformatics

关 键 词： 鸡硒蛋白 硒代半胱氨酸插入序列元件 结构与功能 组织表达谱 进化关系

领　　域： [生物学] [农业科学] [农业科学] [农业科学]