作　　者： ; ; ; ; ; ; ;

机构地区： 广西科学院

出　　处： 《食品与发酵工业》 2014年第2期94-98,共5页

摘　　要： 为了提高丙酮丁醇梭菌对分子氧的耐受能力,降低厌氧发酵环境,构建了超氧化物阻遏蛋白(PERR)基因敲除的工程菌株。应用Ⅱ组内含子敲除系统,PCR克隆perR-Targetron基因与载体连接构建敲除质粒pSYperR,电转化丙酮丁醇梭菌C.acetobutylicum ATCC 824,PCR筛选验证获得突变菌株C.acetobutylicum ATCC 824-ΔperR,采用摇瓶发酵对其突变菌株进行发酵性能研究。结果表明:静止状态发酵丁醇C.acetobutylicum ATCC824-ΔperR比C.acetobutylicum ATCC 824丁醇产量提高7.89%,摇床转速为200 r/min时,C.acetobutylicum ATCC824-ΔperR的丁醇产量是C.acetobutylicum ATCC 824的3.34倍。研究表明,通过Ⅱ组内含子敲除系统,构建的C.acetobutylicum ATCC824-ΔperR在发酵过程中降低了氧分子的伤害,不需要严格的厌氧条件,从而降低发酵成本。 In order to reduce the anaerobic fermentation environment and enhance the tolerance of molecular oxy- gen, a Clostridium acetobutylicum mutant with peroxide regulon repressors （perR） gene in-frame deletion was con- structed through Group II Intron knock-out recombination. The effect of perR knock-out on fermentation characteris- tics of the mutant was investigated by shake-flask fermentation experiments. The fermentation results showed that C. acetobutylicum ATCC824-AperR could enhance the yield of butanol with 7.89% by the stationary state fermentation compared with the C. acetobutylicum ATCC 824. The yield of butanol of C. acetobutylicum ATCC 824-AperR was 3.34 times that of C. acetobutylicum ATCC 824 when the rotate speed was 200 r/rain. Results showed that C. acetobutylicum ATCC 824-AperR constructed by group II introns knock out system could reduce molecular oxygen damage in the process of fermentation, thus reduce fermentation costs.

关 键 词： 丙酮丁醇梭菌 组内含子 丁醇 敲除

领　　域： [生物学]