作　　者： ; ; ; ; ;

机构地区： 华南农业大学农学院草业科学系

出　　处： 《草业科学》 2013年第12期1953-1959,共7页

摘　　要： 本研究从野生狗牙根中分离出的内生固氮菌中,选择固氮酶活性最高、中等及最低3种类型的内生固氮菌,采用3种接种方法,即菌液浸泡、菌液浸泡+浇灌菌液6次及菌液浸泡+浇灌菌液12次,给天堂419杂交狗牙根(Cynodon dactylon×C.transvaalensis cv.Tif-419)接种,并进行施尿素处理和不施尿素对照盆栽试验,探讨内生固氮菌的接种方法及效果。结果表明,固氮酶活性中等的菌液对杂交狗牙根地上部内生固氮菌固氮酶活性的影响大于其它接种菌液和对照;菌液浸泡的接种方法对天堂419杂交狗牙根地上部内生固氮菌固氮酶活性的影响大于菌液浸泡+浇灌菌液6次和菌液浸泡+浇灌菌液12次接种方法以及对照。菌液浸泡方法适用于植物内生固氮菌的接种,但接种方法对菌群密度的影响差异不显著;尿素处理对接种杂交狗牙根地上部内生固氮菌固氮酶活性及菌群密度的影响不显著。 A study was conducted to evaluate the efficiency of entophytic diazotrophs inoculation. Three representative types of endophytic diazotrophs with the highest, moderate and the lowest nitrogenase activity were selected out from endophytic diazotrophic bacteria isolated from wild Cynodon dactylon. T-419 hybrid bermudagrass （Cynodon dactylon X Cynodon transvaalensis） were inoculated with these three representative bacteria using three inoculation methods （microbial soaking, microbial soaking＋irri- gating bacteria 6 times and microbial soaking＋irrigating bactera 12 times）. After inoculation, the plants were planted in pots with urea and no urea as control to examine the efficiency of inoculation methods and the response of T-419 hybrid bermudagrass. The results indicated that Bm bacteria liquid with moderate nitrogenase activity had more influence on nitrogenase activity of aboveground parts of T-419 hybrid ber- mudagrass compared with the other inoculated bacteria and the control. The inoculation method of micro- bial soaking is most effective method compare with the other two which induced higher nitrogenase activity of aboveground parts of T-419 hybrid bermudagrass. It was concluded that microbial soaking is optimal for inoculation of endophytic diazotrophs with hybrid bermudagrass. However, the effects of inoculation methods on density of endogenous azotobacter nitrogenase were not significant. Urea treatment has no sig- nificant influence on endogenous azotobacter nitrogenase activity and density of bacteria of aboveground parts of T-419 hybrid bermudagrass.

关 键 词： 杂交狗牙根 内生固氮菌 固氮酶活性 接种

领　　域： [农业科学] [农业科学] [农业科学]