作　　者： ; ; ; ; ; ;

机构地区： 军事医学科学院卫生学环境医学研究所天津市环境与食品安全风险监控技术重点实验室

出　　处： 《生态毒理学报》 2013年第5期757-762,共6页

摘　　要： 为研究新型阻燃剂磷酸三（2，3-二氯丙基）酯（TDCPP）的毒性效应，以稀有绚鲫（Gobiocyprisrufus）为实验生物，采用半静态实验方法，分别进行96h和28d的染毒，研究TDCPP对稀有绚鲫的急性毒性和慢性毒性效应，并通过对稀有觞鲫脑组织中与神经纤维的生长、发育、轴突再生等相关基因mRNA表达量的检测，初步探讨了TDCPP的神经毒性作用。结果表明：TDCPP对稀有觞鲫的96h．LC50为2．99（220～3．38）mg·L^-1，根据化学物质对鱼类毒性分级标准TDCPP属于剧毒性。经0．9、1．5、2．1和2．7mg·L^-1TDCPP染毒28d，与对照组相比，暴露组稀有朐鲫肝脏及脑组织中SOD和GSH—Px的活性均受到抑制，且随着TDCPP的暴露浓度增加，其抑制作用显著增强；暴露组稀有觞鲫脑组织中与神经纤维的生长、发育相关的微管蛋白α/β、神经丝NF—M以及关键蛋白GAP-43等基因的mRNA表达量，与对照相比均发生显著下调。可知，TDCPP暴露可以诱发稀有绚鲫神经毒性作用。 Toxicological effects of tri（2,3-dichloroprophyl） phosphate （TDCPP） on rare minnow （Gobiocypris rams） were investigated using the semi-static bioassay, including 96 h acute toxicity and 28 d chronic toxicity. We also explored some special gene expression levels such as NF-M, GAP-43, Tubulin et/13, which resulted in neurotoxicity of TDCPP. Results showed that the 96 h-LC50 value of TDCPP to rare minnow was 2.99 （2.20 ~ 3.38） mg·L^-1. According to the criteria for evaluating and grading toxicity of chemicals to fishes, the toxicity of TDCPP should be the hypertoxicity level. The activities of SOD and GSH-Px in both liver and brain tissues of rare minnow were inhibited in comparison with the control, after exposure to 0.9, 1.5, 2.1 and 2.7 mg·L^-1 TDCPPfor 28 d. The inhibition strengthened as TDCPP concentration increased. Furthermore, the expressions of spe- cific genes in brain tissue involved in neurotrophic factor, such as NF-M, GAP-43 and microtubulin α/βwere significantly down-regulated compared with the control. It is indicated that TDCPP may induce the neurotoxic effect on rare minnow.

关 键 词： 磷酸三 二氯丙基 酯 稀有鮈鲫 毒性效应 基因表达

领　　域： [环境科学与工程]