作　　者： ; ; ; ; ; ;

机构地区： 宁波大学信息科学与工程学院

出　　处： 《光电子．激光》 2013年第11期2226-2232,共7页

摘　　要： 由于环境光照的影响,显微镜镜片组在对同一目标成像和计算它们的清晰度时会产生较大偏差。本文基于暗通道先验方法,提出了显微图像光照分离和目标定位模型,进而实现了一种对环境光照影响具有鲁棒性的清晰度计算方法。首先通过计算暗通道得到传输衰减,接着通过分析传输衰减得到显微图像目标区域和光照分离后图像,最后计算目标图像清晰度。实验结果表明,本文方法能有效抑制光照对图像清晰度计算的影响,归一化方差的抑制量最大达到85%,可用于显微镜镜片组的合焦自动检测。 Because of the impact of ambient illum ination,there may exist large deviation in calculating micro-image definitio n according to the traditional definition estimation methods,when the microscope lens group is used to capture micro-image to the same target.The impact may lead to that the assessed micro-image′s definitions fluctuate fiercely.In order to eliminate the effect of ambient illumination,an i llumination separated model and a target object location model are proposed based on the dark channel prior.Furth ermore,an illumination separated model is adopted and a new micro-image definition assessment method is given,wh ich is robust to ambient illumination.Transmission attenuation of the light is first obtained by computi ng dark channel of micro-image, target areas of micro-image are located and the illumination separated image is obtained by analyzing the transmission attenuation,and finally the micro-image definition is more effici ently assessed.Experiment is performed to verify the efficiency of the proposed method.The experimental results show that the proposed method can efficiently suppress the impact of ambient illumination in the proces s of computing micro-image definition,and as a result,the maximal normalized variance is suppressed with 85% with the proposed method. Moreover,the proposed method can be used in the in-focus auto-detecting of mi croscopy lens group.

关 键 词： 光照分离 暗通道先验 归一化方差 清晰度 显微图像

领　　域： [机械工程] [机械工程] [机械工程]