作　　者： ; ; (马效鹏）;

机构地区： 西北农林科技大学园艺学院

出　　处： 《西北农业学报》 2013年第10期131-134,共4页

摘　　要： 利用分子标记技术鉴定"秋白80"大白菜杂种一代的纯度。用41对SSR引物对"秋白80"正反交及其亲本混合池DNA模板进行扩增,从中筛选出1对可将亲本和杂交种有效区分开的引物BrID111757,其对杂交种扩增产生亲本互补的特征带,上述条带均在杂种一代中出现。用这对引物对100个"秋白80"的幼苗进行纯度鉴定,发现分子标记鉴定结果与田间调查结果一致。因此,筛选出的SSR引物BrID111757可用于大白菜品种"秋白80"的纯度鉴定。 Molecular marker was used to identify the F1 hybrid seed purity of commercial heading Chi- nese cabbage（Brassica campestris L. ssp. pekinensis） cultivar ＂Qiubai 80＂. Firstly, one pair of SSR primers, BrID111757, was selected from total 41 pairs which were used to amplify the genome DNA of ＂Qiubai 80＂ and its parents. The SSR primer can amplify a polymorphic product between the par- ents and their progenies, all of them were appeared in identification of the ＂Qiubai 80＂ F1 hybrid. Then, the SSR primer pair was chosen to test 100 seedlings of ＂Qiubai 80＂. The results of molecular marker identification were consistent with the field identification. The study suggested that the SSR primers selected were feasible for identifying hybrid seeds purity of ＂Qiubai 80＂.

关 键 词： 大白菜 秋白 纯度鉴定

领　　域： [农业科学]