作　　者： ; ; ; ; ; ; ;

机构地区： 华南农业大学食品学院

出　　处： 《食品科学》 2013年第20期203-206,共4页

摘　　要： 建立分散固相萃取-高效液相色谱-二极管阵列检测器分析茶饮料中吡虫啉和啶虫脒残留的方法。样品经乙酸乙酯-环己烷(5:5,V/V)萃取,25mgN-丙基乙二胺(PSA)+25mg石墨化炭黑(GCB)分散固相萃取净化。采用Agilent ZORBAX-XDB C18色谱柱分离,乙腈和水洗脱。在0.05、0.10mg/L和0.50mg/L加标水平上,吡虫啉和啶虫脒的回收率分别为93.3%～102.5%、95.0%～112.7%,相对标准偏差(RSD)分别为2.2%～2.9%、3.0%～5.1%。吡虫啉和啶虫脒的检测限分别为0.004、0.001mg/L,定量限分别为0.010、0.006mg/L。方法精密度较高,重复性较好。 A rapid analytical method based on high-performance liquid chromatography with diode array detection was developed for the simultaneous determination of imidacloprid and acetamiprid in green tea infusion. The target analytes were extracted with ethyl acetate-cyclohexane （5：5, V/V）, and the extracts were cleaned up by dispersive solid phase extraction with 25 mg of primary secondary amine （PSA） plus 25 mg of graphitized carbon black （GCB）. The recovery rates at three spiked levels of 0.05, 0.1 mg/L and 0.5 mg/L were 93.3% to 102.5% for imidacloprid and 95.0% to 112.7% for acetamiprid, and the relative standard deviations were 2.2% to 2.9% and 3.0% to 5.1%, respectively. The limits of detection （LOD） of imidacloprid and acetamiprid were 0.004 mg/L and 0.001 mg/L, and the limits of quantification （LOQ） were 0.010 mg/L and 0.006 mg/L, respectively.

关 键 词： 吡虫啉 啶虫脒 茶饮料 分散固相萃取 高效液相色谱

领　　域： [理学] [理学]