作　　者： ; ; ; ; ;

机构地区： 华南农业大学林学院

出　　处： 《Agricultural Science & Technology》 2013年第9期1335-1343,共9页

摘　　要： [目的]了解干旱胁迫对楸树(Catalpa bungei)无性系叶片渗透调节物质的影响。[方法]采用盆栽塑料薄膜封盆法,通过土壤含水量与叶水势变化对干旱胁迫程度进行划分,并研究5个楸树无性系在不同干旱程度下叶片渗透调节物质(游离脯氨酸、可溶性糖及可溶性蛋白)含量的变化。[结果]通过土壤含水量与叶水势的变化,将干旱胁迫过程分为正常(CK)、轻度(LD)、中度(MD)和重度(SD)干旱,其区间为正常干旱(土壤含水量97.49%,叶水势-0.54MPa)、轻度干旱(土壤含水量59.96%,叶水势-1.28MPa)、中度干旱(土壤含水量34.19%,叶水势-2.32 MPa)、重度干旱(土壤含水量14.52%,叶水势-2.99 MPa);5个楸树无性系土壤含水量与叶水势拟合关系均以指数拟合最佳,平均R2达到0.989 3(P<0.001);5个楸树无性系叶片游离脯氨酸含量随干旱胁迫程度的加剧显著上升(P<0.01),其中无性系015-1和无性系7080在SD下分别达到正常状态的34.39倍和33.41倍,达极显著水平(P<0.001);无性系1-3则在LD时快速上升,迅速达(855.46±227.52)μg/g Fw;叶片可溶性蛋白含量表现出不同趋势,无性系7080在各阶段均最低,正常状态仅为(1.644±0.137)mg/g Fw,而无性系1-3在CK时可溶性蛋白含量较高,在LD时迅速下降,体现出不同的响应模式。[结论]5个楸树无性系叶片渗透调节物质对干旱胁迫均有一定的响应,以游离脯氨酸为最主要渗调物质,可溶性蛋白为辅,未见可溶性糖有显著贡献;其中无性系7080在各渗透调节物质含量变化上表现出较高的能力,体现出较好的抗旱性,而无性系1-3则对于干旱胁迫响应最迅速。 [Objective] This study aimed to investigate the effects of drought stress on osmotic regulation substances of Catalpa bungei clones. [Method] Potting experimental was carried out with plastic film sealing method, to differentiate the degrees of drought stress based on changes in soil water content and leaf water potential and investigate variations in contents of osmotic regulation substances（free proline,soluble sugar and soluble protein） in leaves of five Catalpa bungei clones under different degrees of drought stresses. [Result] According to changes in soil water content and leaf water potential, the whole process of drought stress was divided into normal level（CK, with soil water content of 97.49% and leaf water potential of-0.54MPa）, light drought（LD with soil water content of 59.96% and leaf water potential of-1.28 MPa）, mediate drought（MD with soil water content of 34.19% and leaf water potential of-2.32 MPa） and severe drought（SD with soil water content of 14.52%and leaf water potential of-2.99 MPa）. The soil water content and leaf water potential of five Catalpa bungei clones reached the highest correlation in exponential fitting, with an average R2of 0.989 3（P0.001）. The free proline content in leaves of five Catalpa bungei clones increased rapidly with the increasing degree of drought stress（P 0.001）. To be specific, free proline contents of Catalpa bungei clones015-1 and 7080 were 34.39 and 33.41 times of the normal level under severe drought conditions, which reached an extremely significant level（P0.001）; the free proline content of Catalpa bungei clone 1-3 rapidly increased to（855.46±227.52） μg/g Fw under light drought conditions. The soluble protein content in leaves showed different variation trends. To be specific, the soluble protein content of Catalpa bungei clone 7080 was the lowest at various drought stages and reached（1.644 ±0.137）mg/g Fw under normal conditions; the soluble protein content of Catalpa bungei clone 1-3 was relatively high under norm

关 键 词： 干旱胁迫 楸树无性系 渗透调节物质

领　　域： [农业科学]