作　　者： ; ; ; ; ; ; ;

机构地区： 华南农业大学农学院

出　　处： 《西北植物学报》 2013年第9期1768-1774,共7页

摘　　要： 采用SSR分子标记对来自广东省5个县野生大豆居群的遗传多样性进行了分析,为广东野生大豆资源保护和利用提供依据。结果显示：（1）5个野生大豆居群在60个SSR位点共检测出263个等位变异,同一位点上等位基因数目最多为10个,最少为2个,平均为4.38个;不同群体中特有等位基因数不同,其中连州（LZ）和南雄（NX）野生大豆的特有等位基因数目较多,分别为19个和16个;Shannon指数（I）变化范围为0.162~2.174,期望杂合度（He）的变化范围为0.073~0.899。（2）广东连州（LZ）野生大豆居群的遗传多样性最高,而仁化（RH）野生大豆的遗传多样性最低,二者的Shannon指数（I）分别为0.811、0.113;群体分子方差（AMOVA）分析结果揭示,居群间变异占51%,群体内变异占49%,且仁化居群与其他居群间基因流较小。（3）依据遗传距离可将连州（LZ）和连南（LN）聚类为一类,乳源（RY）和南雄（NX）为一类,仁化（RH）单独为一类。研究表明,广东不同野生大豆居群间遗传多样性差异较大,而且居群内基因类型多,其中连州（LZ）和乳源（RY）野生大豆居群的遗传多样性较高,证明广东野生大豆群体保存了丰富的基因资源。 Genetic diversities of 5 wild soybean populations from Guangdong were examined by SSR.The results showed that：（1）There were 263 alleles at 60 SSR loci,varying from 2 to 10 with an average of 4.38 alleles for each locus.Lianzhou and Nanxiong populations had higher unique alleles,each with 19 and 16.Shannon diversity indexes varied from 0.162 to 2.174,while the expected heterozygosity（He）ranged from 0.073 to 0.899.（2）Lianzhou populations had the highest genetic diversity with Shannon diversity index 0.811 among 5 wild soybean populations in Guangdong,while Renhua population had the lowest genetic diversity with Shannon diversity index 0.113.AMOVA analysis results indicated that there is 51% variance among the populations and 49% in populations.Renhua population（RH）had lower gene flow with the other populations.According to genetic distance the 5 populations were divided into three groups：Lianzhou and Liannan as a group,Ruyuan and Nanxiong as a group,Renhua only for a group.The results indicated that there were different levels in genetic diversity among populations and within population,and Lianzhou and Ruyuan populations had higher genetic diversity than others.

关 键 词： 遗传多样性 野生大豆

领　　域： [生物学]