作　　者： ; ;

机构地区： 华南农业大学

出　　处： 《农业生物技术学报》 2000年第1期67-70,共4页

摘　　要： 在田间和温室筛选的基础上，选取根构型对低磷适应性变化和磷效率均具有显著差异的菜豆基因型ＤＯＲ３６４和Ｇ１９８３３为亲本材料，发展了Ｆ_５代重组自交系遗传群体，以基部侧根（以下简称基根）在生长介质中各层的分布、浅根数目以及基根平均生长角度等根构型参数为指标，分析了根构型性状对低磷胁迫适应性变化的遗传特性，发现该性状是一个典型的数量遗传性状。我们进一步应用分子标记技术（ＲＦＬＰ、ＲＡＰＤ、ＡＦＬＰ）对控制其中的一个重要性状（基根平均生长角度）的数量性状座位（ＯＴＬｓ）的进行了分子图谱定位，定位到了该性状对低磷胁迫适应性反应的一些ＱＴＬｓ，并且发现其中部分ＱＴＬｓ与控制菜豆田间磷吸收效率的ＱＴＬ连锁，从而从分子水平上证实菜豆根构型性状与磷吸收效率密切相关，为通过分子标记辅助选择（ＭＡＳ）改良菜豆的磷效率提供了理论基础。 Based on the results of field and greenhouse studies, two parental genotypes, DOR364 and G19833, which are contrasting in P efficiency and adaptive changes of root architecture to P availability, and their eighty-seven recombinant inbred lines (RILs) of F_5 generation were studied in the paper Pouch system. Root architectural traits were quantitatively measured for the parental genotypes and the RILs. Root architecture as indicated by shallow basal root number, average basal root growth angle, absolute basal root length in the top layers and some other parameters was very contrasting in the parents, the efficient genotypes being more plastic gravitropically hence having a shallower root system. Such a difference was inherited to the progenies of the RTLs in the F_5 generation, which showed continuous segregation of these traits. The phenotypic data were then used to detect QTLs associated with these root traits by molecular marker analysis (RFLP, PAPD and AFLP), with focus on the basal root growth angle. A number of loci conferring the basal root growth angle have been identitied in linkage groups. At least one of these QTLs was linked to those conferring P uptake efficiency under field conditions. We conclude that 1) root architecture of common beau is a quantitatively in heritable trait; 2) root architecture of common beau is closely related to P uptake efficiency under field conditions; 3) the QTLs conferring root architecture identified in this study should be helpful to improve P efficiency of common beau via marker assisted se lection.

关 键 词： 根构型 基根平均生长角度 菜豆 低磷胁迫

领　　域： [农业科学] [农业科学]