作　　者： ; ; ; ; ; ; ; ; ; ; ;

机构地区： 广西大学

出　　处： 《黑龙江畜牧兽医》 2013年第10期4-6,176,共4页

摘　　要： 为了研究GLUT 3的功能,试验根据GenBank中公布的牛、人等物种的GLUT 3核苷酸序列设计、合成1对引物,以水牛卵巢组织总RNA为模板,采用RT-PCR法扩增出GLUT 3成熟蛋白编码cDNA序列,将此扩增产物克隆入pMD 18-T载体,进行PCR、双酶切鉴定及序列测定与分析。结果表明:扩增的水牛GLUT 3基因CDS序列长为1 701 bp,经相关生物软件预测,该基因编码494个氨基酸。水牛GLUT 3基因ORF序列与牛、山羊、人、大鼠、猪同源性分别为100%、100%、99%、94%和81%;蛋白序列与牛、山羊、人、大鼠、猪同源性分别为100%、99%、99%、99%和97%。说明GLUT 3是一组在进化上高度保守的蛋白质。 To study the function of GLUT3, a pair of primers was designed based on the nucleotide sequences of GLUT3 gene from bovine, human and other species published in Genbank. The total RNA from ovarian tissue in swamp buffalo was used as a template, and then the GLUT3 cDNA encoding the mature protein was amplified using reverse transcription polymerase chain reaction （RT- PCR）. The amplified product was cloned into pMD 18 -T vector, and then was used for PCR, the identification of double enzymes digestion, and sequencing analysis. The results showed that the coding sequence length of the amplified GLUT 3 gene in swamp buffalo was 1485 bp, encoding 494 amino acids predicted by biology - related software. The ORF sequence of the GLUT 3 exhibited high homology with cattle （ 100% ）, sheep （ 100% ）, human （99%）, mouse （94%） and pig （81%）, respectively. Its protein sequence exhibited high homology with cattle （ 100% ）, sheep （99%）, human （99%）, mouse （99%） and pig （97%）, respectively. The results indicate that GLUT3 belongs to a group of highly conserved proteins in evolution.

关 键 词： 葡萄糖转运体 水牛 序列分析

领　　域： [农业科学] [农业科学]