作　　者： ; ; ;

机构地区： 华南农业大学理学院生物材料研究所

出　　处： 《食品科学》 2013年第18期87-91,共5页

摘　　要： 以竹叶为原料提取叶绿素,叶绿素再经过铜代、脱脂等步骤制备油溶性叶绿素铜。对影响产品质量指标的铜代关键工艺过程,在单因素试验的基础上,利用Box-Behnken Design试验设计原理,选择三因素三水平进行铜代工艺条件响应面法分析优化。结果表明:铜代过程最佳条件为加铜量为理论值的2.2倍、反应温度60℃、反应时间75min。在此条件下,油溶性叶绿素铜得率4.051mg/g,检验结果主要指标符合食品添加剂的规定。 Chlorophyllin was extracted from bamboo leaves and used to prepare oil-soluble copper chlorophyllin by copper replacement and degreasing. The procedure of copper replacement critical for the quality of copper chlorophyllin was optimized by response surface methodology based on a three-variable, three-level Box-Behnken design. The optimal conditions for copper replacement were established as follows： the amount of copper was 2.2-fold of the theoretical amount and the reaction was allowed to proceed for 75 min at 60 ℃. Under these conditions, the yield of copper chlorophyllin was 4.051 mg/g. The main quality indicators of the final product obtained met the requirements for use as a food additive.

关 键 词： 竹叶 叶绿素 叶绿素铜 制备工艺 响应面分析

领　　域： [轻工技术与工程] [轻工技术与工程]