作　　者： ; ; ; ; ; ;

机构地区： 暨南大学生命科学技术学院化学系

出　　处： 《分析化学》 2013年第9期1413-1417,共5页

摘　　要： 以金纳米棒为荧光探针,基于H2O2对金纳米棒的氧化作用,导致金纳米棒的荧光光谱发生变化,建立一种对H2O2检测以及评价多酚化合物抗氧化能力的新方法。采用透射电镜(TEM)和局域表面等离子共振(LSPR)光谱对金纳米棒进行表征。在最佳实验条件下,H2O2氧化金纳米棒使其719 nm处的荧光峰下降,且H2O2浓度在2.0×10#6～3.5×10#5mol/L范围内与荧光强度呈线性关系,检出限为8.0×10#7mol/L(S/N=3)。进一步在该体系中加入多酚化合物,利用其具有清除活性氧的能力,可使金纳米棒的荧光信号的恢复,从而评价化合物的抗氧化能力。以芒果苷(MGF)、白藜芦醇(RSV)、瑞香素(DAP)研究此体系对活性氧的清除作用,抗氧化能力依次为:MGF>RSV>DAP,此结果与DPPH法的检测结果相符。 Gold nanorods were used as fluorescent probe. Based on the oxidation of hydrogen peroxide to gold nanorods, resulting in the change of fluorescence spectrums of gold nanorods, we established a new method for the detection of hydrogen peroxide and evaluation of the antioxidant activity of polyphenolic compounds. The gold nanorods were characterized by transmission electron microscopy （TEM） and localized surface plasmon resonance （LSPR） spectrum. Under the optimal conditions, gold nanorods were oxidized by hydrogen peroxide, causing the decrease of fluorescence peaks at 719 nm, and the fluorescence intensity responded linearly to the concentration of hydrogen peroxide form 2.0×10^-6 mol/L to 3.5 × 10^-5 mol/L with a detection limit of 8.0×10^-7 mol/L. Utilizing their ability of scavenging active oxygen, polyphenolic compounds, such as mangiferin （MGF） , resveratrol （RSV） and daphnetin （DAP） , were futher used to recover the fluorescence signals of gold nanorods, which could evaluate the antioxidant ability of polyphenolie compounds. The results of experiments revealed that the antioxidant ability was MGF 〉 RSV 〉 DAP, which was consistent with the method of diphenyl-l-picrylhydrazyl （DPPH）.

关 键 词： 金纳米棒 荧光 过氧化氢 多酚化合物 抗氧化

领　　域： [理学] [理学]