作　　者： ; ; ; ; ; ;

机构地区： 聊城大学生命科学学院

出　　处： 《食品研究与开发》 2013年第15期5-8,共4页

摘　　要： 研究中国蜂胶和巴西蜂胶乙醇提取物对过氧化氢(H2O2)诱导的大鼠BRL肝细胞损伤的保护功效。方法:BRL肝细胞经H2O2预孵1 h后,分别经DMEM培养液、中国蜂胶乙醇提取物(EECP)、巴西蜂胶乙醇提取物(EEBP)处理24 h,分别测定细胞存活率、细胞凋亡率以及细胞培养液中谷丙转氨酶(ALT)、谷草转氨酶(AST)、超氧化物歧化酶(SOD)以及丙二醛(MDA)的变化。与H2O2组相比,EECP和EEBP均显著提高H2O2损伤后BRL肝细胞的存活率,抑制细胞的凋亡;降低细胞培养液中ALT和AST水平;提高SOD的活性。尽管中国蜂胶和巴西蜂胶化学组分不同,但中国蜂胶和巴西蜂胶均具有较好的保肝功效,其机制与提高细胞存活率、抑制细胞凋亡以及降低细胞内AST、ALT水平,升高SOD活性有关。 To investigate the protective effect of ethanol extract of Chinese propolis （EECP） and ethanol extract of Brazilian propolis （EEBP） on the injury of rat BRL hepatocytes induced by hydrogen peroxide （H202） in vitro. BRL hepatocytes were administrated by H202 for 1 h, then treated by DMEM culture, EECP and EEBP respectively for 24 h. Cell viability, apoptosis rate and the levels of alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, super oxygen dehydrogenises （SOD） and malondialdehyde （MDA） in supernate were measured 24 hours later, cell viability of BRL hepatocytes induced by H202 was significantly increased by EECP and EEBP for 24 h; the apoptosis rate was obviously depressed. The levels of ALT and AST supernate were depressed by EECP and EEBP, and the SOD activity was increased obviously by EECP and EEBP. Chinese propolis and Brazilian propolis also have good protective effects on the injury of hepatocytes, and the possible mechanisms are related to increasing the cell viability and SOD activity, depressing apoptosis rate and the levels of ALT and AST.

关 键 词： 中国蜂胶 巴西蜂胶 肝损伤 过氧化氢

领　　域： [生物学]