作　　者： ; ; ; ; ; (邱齐骏）; (徐毛喜）;

机构地区： 南昌大学生命科学学院生物科学系

出　　处： 《南昌大学学报（理科版）》 2013年第3期259-261,271,共4页

摘　　要： 池蝶蚌精子具有子弹形头部及细长均匀鞭毛尾,属鞭毛型。全长为254.91±20.03μm;头部子弹形,长为32.33±2.15μm,宽为16.60±3.29μm;鞭毛细长均匀,长为216.67±19.73μm。为了更好的计算池蝶蚌精子的存活率,本研究采用曙红Y染色的方法,观察了精子在生理盐水、池塘水和蒸馏水中的存活时间及运动方式。结果显示,精子在生理盐水中的存活时间最长,可达6h;其次是蒸馏水,约为4.5h;最短存活时间是在池塘水中,约为4.0h。池蝶蚌精子的主要运动方式有快速直线游动、弧线运动和原地转动3种方式。快速直线游动的精子活力最高,其次是弧线运动,精子活力降低后进行原地转动。研究的结果对池蝶蚌家系的建立起重要作用。 In the current study,the movement and survival time of the sperms from Hyriopsis schlegelii in three kinds of solution were investigated by using a eosin-Y dye method.It was observed that the sperm had a bullet-shaped head and a flagella tail,the overall length was 254.91± 20.03μm,the head length was 32.33±2.15μm,the head width was 16.60±3.29μm and the length of the flagella was about 216.67±≈19.73 μm.The longest survival time of the sperms was 6 h in the normal saline,whereas the shortest time was in pond water,being about 4.5h.There were three cases on the motion modes of sperm：fast linear motion,curved rotation and swaying movement.There results were very important for us to determine the establishing families from Hyriopsis schlegelii.

关 键 词： 池蝶蚌 曙红 染色 精子存活率 精子活力

领　　域： [生物学]