作　　者： ; ;

机构地区： 中山大学化学与化学工程学院

出　　处： 《应用与环境生物学报》 2000年第5期477-482,共6页

摘　　要： 测定了PseudomonasfluorescensFS1的邻苯二甲酸酯降解酶对邻苯二甲酸二丁酯的降解特性 ;提出了邻苯二甲酸二丁酯酶促降解的途径 .PseudomonasfluorescensFS1细胞中的颗粒部分、溶液部分对邻苯二甲酸二丁酯都具有降解作用 ,PseudomonasfluorescensFS1的邻苯二甲酸酯降解酶属胞内酶 ;PseudomonasfluorescensFS1的邻苯二甲酸酯降解酶对邻苯二甲酸二丁酯的的最适酸度为pH 6 .5～ 8.0 ,θ =2 5～ 35℃ ,米氏常数Km 为 2 15 .0 5nmol/mL .在好氧条件下 ,邻苯二甲酸二丁酯与PseudomonasfluorescensFS1的邻苯二甲酸酯降解酯酶作用 ,形成邻苯二甲酸单酯和邻苯二甲酸后 ,可进一步降解成苯甲酸、对羟基苯甲酸和酚 ,最终转化为CO2 和H2 O .图 9表 1参 Degradative characteristics and the metabolic pathway of di n butyl phthalate were determined by the enzyme extracted from Pseadomonas fluorescens FS1 isolated from the activated sludge at a petrochemical factory . The results showed that the PAEs degrading enzyme, mainly located in soluble part of cytoplasm, was an intracllular enzyme. The optium temperature and pH of the PAEs degrading enzyme activity to di(2 ethylhexyl)phthalate were 20～35℃ and 6.5～8.0, respectively .The Michaelis mentn's contant ( K m) of Di(2 ethyl hexyl)Phthalate enzymatic degradation was about 215.05 nmol/mL at 30℃. The enzymatic metabolism of di n butyl phthalate under aerobic conditions starts by PAEs degrading enzyme to monoester, further enzymatic degradation of monoester proceeds via phthalic acid by benzoate, 4 hydroxy benzoic acid and phenol, and then to CO 2 and H 2O. Fig 9, Tab 1, Ref 8

关 键 词： 邻苯二甲酸二丁酯 微生物 酶降解 代谢途径

领　　域： [环境科学与工程] [环境科学与工程]