作　　者： ; ; ; ; ; ; ; ; ; (徐鹏）;

机构地区： 深圳出入境检验检疫局

出　　处： 《动物医学进展》 2013年第9期6-11,共6页

摘　　要： 为监测进境冻畜禽产品中受产超广谱β-内酰胺酶（ESBL）大肠埃希茵污染的情况，采用ESBL显色平板初筛结合Vitek2compact确证及药敏测试的方法，经过与美国临床实验室标准化研究所（CLSI）推荐方法（M100-S22）进行比对，并且通过对249份进境冻畜禽产品进行了检测。结果表明，采用的方法与CLSI推荐方法相比，具有快速、准确、操作简便和特异性好等优点，有良好的推广价值。同时，经检测获得产ESBL大肠埃希茵18株，占样品总量的7．23％。所获菌株对氨苄西林、头孢唑啉和头孢曲松高度耐药，多重耐药率为16．67％～38．89％，为检验检疫部门评估进境冻畜产品中受产ESBL大肠埃希菌污染的情况提供了科学数据。 To investigate the prevalence and distribution of drug-resistant of Escherichia coli with ESBL in imported livestock and poultry products, preliminary screening test and confirmatory test metheds recom- mended by CLSI and the chromID ESBL, VITEK 2 Compact were used to detect 249 imported livestock and poultry product samples, among which 18 consecutive Escherichia coli isolates producing the extend- ed-spectrum β-lactamases （ESBL） were collected, accounting for 7.23%. These strains were resistant to ampicillin, cefazolin and ceftriaxone, and showed multi-drug resistance with rates ranging from 16.67%- 38.89%. The chorm ID ESBL and Vitek2 compact systems demonstrated adequate performance to be used for the ESBL screening, as a rapid,accurate, convenient, special method. And this study will provide scien- tific data for inspection and quarantine department to evaluate the Escherichia coli with extended-spectrumβ-1actamases in imported livestock products.

关 键 词： 进境畜禽产品 超广谱 内酰胺酶 大肠埃希菌

领　　域： [农业科学] [农业科学] [农业科学]