作　　者： ; ; ; ; ; ;

机构地区： 西北农林科技大学农学院

出　　处： 《作物学报》 2013年第8期1386-1390,共5页

摘　　要： 为明确普通小麦中国春（CS）–簇毛麦整臂互补易位系T1DS.1VL和T1DL.1VS对农艺特性和加工品质的效应,于2008—2010年在陕西杨凌连续2个生长季对这2个易位系和CS的主要农艺性状和加工品质性状进行了研究,并采用SDS-PAGE法对簇毛麦高分子量麦谷蛋白亚基（HMW-GS）基因进行了进一步的染色体臂定位。结果表明,这2个易位系的抽穗期和成熟期相同,但比CS晚1~2 d,T1DS.1VL其他农艺性状与中国春相似,T1DL.1VS的春季单株分蘖、千粒重和单株粒重显著高于中国春;2个易位系的籽粒蛋白质含量与中国春无显著差异,T1DS.1VL的Zeleny沉淀值、面团形成时间、稳定时间和粉质仪质量指数显著降低;相反,T1DL.1VS的这些性状值显著提高。说明T1DS.1VL易位系对小麦的面团强度有显著的负向效应,而T1DL.1VS易位系显著增强面筋强度。SDS-PAGE分析结果表明,簇毛麦的1VS和1VL上均含有簇毛麦的HMW-GS基因Glu-V1,但该基因在T1DL.1VS中的表达可能强于在T1DS.1VL中。 To understand the effect on agronomic traits and grain processing quality of Chinese Spring（CS）–Dasypyrum villosa compensating Robertsonian translocation lines T1DL.1VS and T1DS.1VL,we conducted a 2-year field trial in Yangling,Shaanxi Province from 2008 to 2011.Some agronomic traits and processing quality characteristics of T1DL.1VS and T1DS.1VL were compared with those of CS,and the high-molecular-weight glutein subunit（HMW-GS） genes of D.villosum were located on chromosome arms using SDS-PAGE method.The heading and maturity dates of both translocation lines were the same,but 1–2 d later than those of CS.Compared to CS,T1DS.1VL had similar other agronomic traits;however,T1DL.1VS showed higher values of spring tillers per plant,1000-grain weight,and grain weight per plant.Although the seed protein contents of both translocation lines were not significantly different from those of CS,T1DS.1VL exhibited significantly lower Zeleny sedimentation value,developing time,stability,and quality index of farinograph than CS;the values of these quality parameters were significantly higher in T1DL.1VS than in CS.Therefore,T1DS.1VL has a significant negative effect on wheat dough strength,and T1DL.1VS significantly increase gluten strength.HMW-GS gene Glu-V1 of D.villosum was located on both of 1VS and 1VL,but the gene expression level might be higher in T1DL.1VS than in T1DS.1VL.

关 键 词： 中国春 簇毛麦 易位系 农艺性状 加工品质

领　　域： [农业科学]