作　　者： ; ; ; ; ; ; ;

机构地区： 南京农业大学

出　　处： 《南京农业大学学报》 2013年第4期77-82,共6页

摘　　要： 利用平板计数、实时荧光定量RT-PCR和PCR-DGGE指纹图谱等方法研究田间条件下连作番茄和辣椒施用生物有机肥(BOF)和常规施肥(CK)的根际土壤微生物组成差异。结果表明:与CK相比,BOF处理的番茄和辣椒产量分别提高了26.0%和19.9%,青枯病发病率分别降低了41.5%和44.7%;对于未发病植株,与CK相比,BOF处理的番茄和辣椒植株根际土壤细菌数量分别增加了62.7%和142.4%,放线菌数量分别增加了32.8%和39.6%,真菌数量分别减少了43.9%和53.1%,病原菌数量分别减少了73.2%和90.1%;对于发病植株,与CK相比,BOF处理的番茄和辣椒植株根际土壤细菌和放线菌数量没有显著差异,但真菌和病原菌数量显著降低。PCR-DGGE分析表明,施用BOF提高了番茄和辣椒植株根际土壤微生物群落结构多样性指数、丰富度指数和稳定性指数。 Microbial flora and Ralstonia solanacearum population in rhizosphere soils of continuous cropping tomato and pepper in field under application of a bio-organic fertilizer(BOF)and conventional management(CK)were studied with focus on the difference of microflora in rhizosphere soil of tomato and pepper by using plate count,real-time quantitative PCR and PCR-DGGE fingerprint techniques.As compared with CK,the BOF treatment increased the yields of tomato and pepper by 26.0% and 19.9% and decreased the bacterial wilt incidence rates of tomato and pepper by 41.5% and 44.7%,respectively.For non-diseased plants,the BOF treatment increased the numbers of bacteria and actinomycetes by 62.7% and 32.8% in rhizosphere soils of tomato and by 142.4% and 39.6% in rhizosphere soils of pepper,and decreased the numbers of fungi and R.solanacearum by 43.9% and 73.2% in rhizosphere soils of tomato and by 53.1% and 90.1% in rhizosphere soils of pepper,respectively,as compared with CK.For diseased plants,no significant difference between the BOF treatment and CK existed in the numbers of bacteria and actinomycetes in rhizosphere soils of both tomato and pepper,but the BOF treatment significantly decreased the numbers of fungi and R.solanacearum in rhizosphere soils of both tomato and pepper as compared with CK.PCR-DGGE analysis showed that application of BOF enhanced the diversity,richness and stability indices of microbial community in the rhizosphere soils of tomato and pepper.

关 键 词： 生物有机肥 根际土壤 微生物区系 青枯病 番茄 辣椒

领　　域： [农业科学] [农业科学]