作　　者： ; ; ; ; ; ;

机构地区： 广东药学院药科学院

出　　处： 《食品科学》 2013年第15期81-86,共6页

摘　　要： 目的:研究茶枝柑皮提取物中不同乙醇体积分数沉淀多糖的分子质量分布以及其对大鼠嗜铬瘤细胞株(PC12)氧化损伤的保护作用。方法:采用不同体积分数乙醇分步醇沉的方法提取茶枝柑皮提取物中的多糖,通过分离纯化、凝胶渗透色谱(GPC)表征多糖分子质量;建立以H2O2诱导细胞氧化损伤模型,测定细胞存活率分析提取物中各醇沉多糖对细胞氧化损伤的保护作用。结果:分步醇沉多糖分子质量随着乙醇体积分数的增加而减小,乙醇体积分数分别为50%、70%和90%所得多糖的重均分子质量(Mw)分别为72059、47433u和18743u。粗多糖和透析处理后的多糖都具有一定的抗氧化活性,但脱色和脱蛋白处理后的多糖基本失去抗氧化活性。结论:茶枝柑皮提取物中多糖的分子质量分布对抗氧化活性有一定影响;多糖的分子组成(例如含有色素和蛋白质)与抗氧化活性存在密切关系。 Objective： To explore the molecular weight of polysaccharides from Citrus reticulate Chachi peel extract and the protective effect of crude and purified polysaccharides against oxidative injury in pheochromocytoma cells （PC 12）. Methods： Polysaccharide was extracted from Citrus reticulate Chachi peel by different ethanol concentration gradients. The molecular weight of purified polysaccharide fractions was measured. The protective effect on PC12 cells was explored with H202- induced cellular oxidative damage model by the survival rate of the cells. Results： Polysaccharides with smaller molecular weights were obtained by increasing the ethanol concentration and the average molecular weights （Mw） of polysaccharides obtained at ethanol concentrations of 50%, 70% and 90% were 70259, 47433 u and 18743 u, respectively. Both crude polysaccharides and purified polysaccharides after dialysis had antioxidant activity. Conclusion： The antioxidant activity of Citrus reticulate Chachi peel extract shows obvious relationships with the molecular weight distribution of polysaccharides or the presence of other molecules such as pigment and protein.

关 键 词： 茶枝柑皮提取物 多糖 分子质量分布 抗氧化活性

领　　域： [轻工技术与工程] [化学工程]