作　　者： ; ;

机构地区： 同济大学

出　　处： 《中国应用生理学杂志》 2013年第4期339-344,共6页

摘　　要： 目的:利用二联苯碘(DPI)合并谷氨酰胺(Gln)补充的干预手段,对过度训练引起的运动性免疫抑制的防护方法进行探讨及机制分析。方法:50只雄性Wistar大鼠随机分为安静组(C)、过度训练组(E)和DPI干预组(D)、Gln补充组(G)及DPI合并Gln补充组(DG),过度训练后进行血浆细胞因子、丙二醛(MDA)、髓过氧化物酶(MPO)的ELISA测定及NADPH氧化酶化学发光法测定;流式细胞技术进行中性粒细胞呼吸爆发和吞噬功能的测定;Western blot半定量测定gp91phox和p47phox的蛋白表达。结果:同对照组比较,过度训练后G组除NO显著上升外其余指标变化与E组同步,但与E组比较,DG组中的NO、CINC浓度则显著降低(P<0.05);D组、G组的呼吸爆发与吞噬功能有上调的趋势,DG组显著性上升(P<0.01);过度训练后E组gp91phox和p47phox的表达均显著上调(P<0.01),除DG组的gp91phox的蛋白表达显著下调(P<0.05)外,其余各组均无显著变化;同E组比较,D组、G组及DG组的gp91phox、p47phox蛋白表达均显著下调(P<0.05)。结论:过度训练时血浆炎性因子、趋化分子、黏附蛋白的表达上调是激活中性粒NADPH氧化酶介导产生ROS的过氧化损伤的潜在因素。DPI合并Gln补充可有效地逆转过度训练引起的中性粒细胞的功能水平。 Objective： To investigate the method and mechanism for exercise-related immunosuppression via the inhibitor of NADPH oxidase diphenyleneiodonium（DPI） and glutamine supplementation and on the function of neutrophils after overtraining.Methods： Fifty male Wistar rats were randomly divided into five groups： a negative control group（C）,an overtraining group（E）,an overtraining ＋ DPI intervention group（D）,an overtraining＋ glutamine supplementation group（G） and combined glutamine ＋ DPI intervention group（DG）.After 36~40 h from the last training,eight rats were randomly selected from each group,and blood was sampled from the orbital vein.ELISAs were used to measure serum cytokine levels and lipid peroxidation in blood plasma.Flow cytometry was used to measure neutrophil respiratory burst and phagocytosis.The activity of NADPH oxidase was assessed by chemiluminescence and the gene expression of gp91phox and p47phox of the NADPH-oxidase subunit was checked by Western blot.Results： Compared with group C,the plasma concentrations of NO increased in group G,and the NO,cytokine-induced neutrophil chemoattractant（CINC） concentrations in group DG increased significantly.The respiratory burst and phagocytosis function of neutrophils were decreased in group E,but in group DG were increased when compared with those of group E.After overtraining the expression of gp91phox and p47phox was up regulated in group E.There were no significant changes in other groups except group DG,in which the expression of gp91phox was down regulated.Compared with group E,the expression of gp91phox and p47phox was up regulated in group D,group G and group DG.Conclusion： The activation of NADPH oxidase is responsible for the production of superoxide anions,which may be related to the decrease in neutrophil function after over training and is the mechanism of exercise-related immunosuppression.The DPI treatment combined glutamine supplementation can reverse the decrease neutrophils function after overtraining in

关 键 词： 过度训练 中性粒细胞 活性氧 呼吸爆发 二联苯碘 谷氨酰胺

领　　域： [文化科学] [文化科学]