作　　者： ; ; ; ; ;

机构地区： 黑龙江八一农垦大学食品学院

出　　处： 《农产品加工（下）》 2013年第7期25-27,共3页

摘　　要： 对球毛壳霉高产木聚糖酶的发酵条件进行研究,通过对发酵温度、发酵时间、初始pH值和接种量4个影响因素的单因素试验,利用DNS酶活测定法测定其酶活,从而确定球毛壳霉产木聚糖酶的最适发酵条件。结果表明,生产木聚糖酶的最佳条件是温度为35℃,初始pH值为6.0,接种量为2%,发酵时间为48 h,在该条件下木聚糖酶活性最高,为1 869.13 U/mL。 The producing xylanase in Chaetomium globosum is studied in this experiment,the single factor experiment in four affecting factors including fermentation temperature,time,initial pH value and inoculation amount and xylanase activity using the DNS method are determined,accordingly,the optimal fermentation condition of producing xylanase are determined.The results show that optimal conditions on xylanase production are temperature for 35 ℃,pH value for 6.0,initial inoculated amount 2%,fermentation time for 48 h,the xylanase activity under this condition is supreme,that is 1 869.13 U/mL.

关 键 词： 球毛壳霉 木聚糖酶 发酵条件

领　　域： [生物学]